Abstract

The effect of pinacidil was studied on calcium-loaded rat heart mitochondria (RHM) in the presence of succinate and rotenone. In experiments with pinacidil, the swelling of these mitochondria increased in media with NH₄NO₃ or K-acetate, but the inner membrane potential (ΔΨ_mito) and the respiration in 3 or 2,4-dinitrophenol-stimulated states of these organelles decreased due to the opening of the mitochondrial permeability transition pore (MPTP) in their inner membrane. These effects were inhibited by cyclosporin A and ADP. It was concluded that the protective effect of pinacidil in the cardiac muscle under ischemia/reperfusion may be associated with both the stimulation of mitochondrial swelling and a decrease in RHM calcium overload resulted in ΔΨ_mito fall due to mild uncoupling effect of pinacidil.

中文翻译：

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松香酮存在下吡那地尔和钙对琥珀酸酯化的大鼠心脏线粒体的影响。

摘要

在琥珀酸和鱼藤酮存在下，研究了吡那地尔对钙负荷大鼠心脏线粒体（RHM）的影响。在使用吡那地尔的实验中，在含有NH ₄ NO ₃或K-乙酸盐的培养基中，这些线粒体的溶胀增加，但这些细胞器的内膜电位（_ΔΨmito）和3或2,4-二硝基苯酚刺激状态的呼吸作用降低。由于其内膜中线粒体通透性过渡孔（MPTP）的开放。这些作用被环孢菌素A和ADP抑制。结论是吡那地尔在缺血/再灌注下对心肌的保护作用可能与刺激线粒体肿胀和降低RHM钙超载导致ΔΨ有关由于吡那地尔的轻度解偶联作用，_mito下降。