The two-factor theory predicts that the acquisition of avoidance responses is dependent on fear reduction; as such, drugs that reduce or increase fear or anxiety states should alter inhibitory avoidance (IA) acquisition. The present experiment used white spaces as aversive unconditioned stimuli in IA in zebrafish. Adult zebrafishes were tested in three experiments: validation of white compartment as aversive in IA; open field test; and effect of antidepressant (fluoxetine, imipramine) and anxiolytic (diazepam, clonazepam). The data show the effectiveness of the white compartment as an aversive stimulus in IA. Antidepressant fluoxetine did not alter and imipramine impairs avoidance acquisition in higher doses. Imipramine also produced a sedative effect in lower doses. Anxiolytic and stimulant drugs facilitated learning at doses which did not impair locomotion, suggesting that pharmacological manipulation of other factors in addition to fear/anxiety can impact aversive learning in zebrafish.

中文翻译：

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白色环境可以用作斑马鱼抑制回避学习中的厌恶刺激物。

两因素理论预测，回避反应的获得取决于减少恐惧感。因此，减少或增加恐惧或焦虑状态的药物应改变抑制性规避（IA）的获得。本实验在斑马鱼的IA中使用空白空间作为厌恶性无条件刺激。在三个实验中对成年斑马鱼进行了测试：在IA中验证白色隔室是否具有厌恶性；野外测试；和抗抑郁药（氟西汀，丙咪嗪）和抗焦虑药（地西p，氯硝西am）的作用。数据显示白色隔室作为IA中厌恶刺激的有效性。抗抑郁药氟西汀没有改变，丙咪嗪会损害大剂量的回避获得。丙咪嗪在低剂量时也产生镇静作用。