This study shows vanadate (V(V)) reduction in a methane (CH₄) based membrane biofilm batch reactor when the concentration of dissolved oxygen (O₂) was extremely low. V(IV) was the dominant products formed from V(V) bio-reduction, and majority of produced V(IV) transformed into precipitates with green color. Quantitative polymerase chain reaction and Illumina sequencing analysis showed that archaea methanosarcina were significantly enriched. Metagenomic predictive analysis further showed the enrichment of genes associated with reverse methanogenesis pathway, the key CH₄-activating mechanism for anaerobic methane oxidation (AnMO), as well as the enrichment of genes related to acetate synthesis, in archaea. The enrichment of aerobic methanotrophs Methylococcus and Methylomonas implied their role in CH₄ activation using trace level of O₂, or their participation in V(V) reduction.

中文翻译：

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在缺氧条件下，膜生物膜间歇反应器中的甲烷氧化与钒酸盐还原反应耦合。

这项研究表明，当溶解氧（O ₂）的浓度极低时，基于甲烷（CH ₄）的膜生物膜间歇反应器中的钒酸盐（V（V））降低。V（IV）是由V（V）生物还原形成的主要产物，大多数产生的V（IV）转化为绿色沉淀。定量聚合酶链反应和Illumina测序分析表明，古细菌甲烷八叠球菌明显富集。元基因组预测分析进一步显示了与反向甲烷生成途径（关键CH _4）相关的基因的富集甲烷中厌氧甲烷氧化（AnMO）的活化机制以及与乙酸盐合成相关的基因的富集。有氧甲烷营养菌甲基球菌和甲基单孢菌的富集暗示了它们在使用痕量O _2的CH ₄活化中的作用，或参与了V（V）的还原。