CRISPR/Cas9 approaches are revolutionizing our ability to perform functional genomics across a wide range of organisms, including the Plasmodium parasites that cause malaria. The ability to deliver single point mutations, epitope tags and gene deletions at increased speed and scale is enabling our understanding of the biology of these complex parasites, and pointing to potential new therapeutic targets. In this review, we describe some of the biological and technical considerations for designing CRISPR-based experiments, and discuss potential future developments that broaden the applications for CRISPR/Cas9 interrogation of the malaria parasite genome.

中文翻译：

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减少疟疾：疟原虫的 CRISPR/Cas9 基因组编辑。


CRISPR/Cas9 方法正在彻底改变我们对多种生物体进行功能基因组学的能力，包括引起疟疾的疟原虫寄生虫。以更高的速度和规模提供单点突变、表位标签和基因删除的能力使我们能够了解这些复杂寄生虫的生物学，并指出潜在的新治疗靶点。在这篇综述中，我们描述了设计基于 CRISPR 的实验的一些生物学和技术考虑因素，并讨论了扩大 CRISPR/Cas9 疟疾寄生虫基因组研究应用的潜在未来发展。