In this study, zebrafish follicles containing stage III oocytes were isolated and incubated in vitro to allow oocyte maturation. A minicell containing the intact oocyte spindle was excised after proteolytic digestion of the micropyle area of the follicle. The minicell was subjected to hypotonic treatment in deionized water for 10 min, and then spread on glass slide for karyotyping. Dyads at metaphase II and sister chromatids at anaphase II are clearly shown. Also, cytochalasin B treatment inhibited chromosomal segregation. This simple and inexpensive chromosome preparation protocol could be readily applicable to other fish species.

中文翻译：

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斑马鱼卵母细胞核型分析的方法。

在这项研究中，包含III期卵母细胞的斑马鱼卵泡被分离并在体外孵育以使卵母细胞成熟。在蛋白水解消化卵泡的微小区之后，切下含有完整卵母细胞纺锤体的小细胞。将小细胞在去离子水中进行低渗处理10分钟，然后铺在载玻片上进行核型分析。清楚地显示了在中期II的染色质和在后期II的染色单体。同样，细胞松弛素B处理抑制了染色体的分离。这种简单而廉价的染色体制备方案可以很容易地应用于其他鱼类。