Sixty traditional leafy vegetables, comprising of mutete (Hibiscus sabdariffa) (n = 20) and omboga (Cleome gynandra) (n = 40) were analysed for fungal, plant and bacterial metabolites using liquid-chromatography-tandem mass spectrometry. No European Union legislated mycotoxins were quantified and no vegetables contained levels above the FAO/WHO limit of 10 mg/kg for cyanogenic potential, suggesting comparative safety regarding regulated mycotoxins and cyanogenic glycosides. Quantified fungal metabolites included averufin and 3-Nitropropionic acid from Aspergillus flavus, beauvericin and equisetin from Fusarium, citrinin and curvularin from Penicillium and altertoxin −1 and tentoxin from Alternaria. Of the plant cyanogenic glycosides, linamarin was quantifiable in 65% of mutete at a maximum of 398 µg/kg but not in omboga, while lotaustralin was quantifiable in both omboga and mutete. The bacterial metabolite nonactin was detected in 27.5% of omboga samples (range: 0.2–7.3 μg/kg). Minimal variation in metabolite patterns was recorded for omboga samples from Oshana and Oshikoto regions.

使用液相色谱-串联质谱法分析了六十种传统的多叶蔬菜，包括无声（Hibiscus sabdariffa）（n = 20）和omboga（Cleome gynandra）（n = 40），以分析真菌，植物和细菌的代谢产物。没有对欧盟立法规定的霉菌毒素进行定量分析，也没有蔬菜含量超过粮农组织/世卫组织对生氰潜力的限制（10毫克/千克），这表明有关受规管的霉菌毒素和生氰苷的比较安全。量化真菌代谢包括来自averufin和3-硝基丙酸黄曲霉，白僵菌素和equisetin从镰孢属，桔霉素和从curvularin青霉和altertoxin -1和tentoxin从黑斑。在植物中的氰化物苷中，亚麻苦参碱在65％的突变体中可定量，最大浓度为398 µg / kg，但在omboga中未定量，而lotaustralin在omboga和Mutute中均定量。在27.5％的omboga样品中检测到细菌代谢物非肌动蛋白（范围：0.2–7.3μg/ kg）。记录了来自Oshana和Oshikoto地区的omboga样品的代谢物模式变化最小。