The aim of this study was to evaluate the immunoexpression of HER-2 in gastric cells of cats infected with Non H. pylori Helicobacter (NHPH) and to investigate an association with the presence of inflammatory infiltrate. Forty-eight paraffin-embedded gastric samples were retrieved from the archives of the Veterinary Anatomic Pathology Laboratory that had previously been shown to be positive for NHPH with the rapid urease test and cytology. Infection by NHPH was confirmed by histopathology using the Warthin-Starry staining. Hematoxylin-eosin stained sections were reviewed to evaluate inflammatory cell infiltrates. Immunohistochemical analysis was done using anti- H. pylori antibody and anti-HER-2 antibody. Molecular analysis was performed by PCR to confirm the presence of Helicobacter. Statistical analysis was performed to determine whether there was an association between the presence of H. Heilmannii and HER-2 expression in gastric samples. All samples were positive for NHPH, by immunohistochemistry, and confirmed by PCR as H. Heilmannii. On histopathologic analysis, 56,3% of the samples had lymphocytes and plasma cells infiltrates, 52,1% of which were mild and 4,2% moderate. The intensity of the inflammatory infiltrate in the gastric mucosa was significantly greater in the complete plasma membrane of parietal cells of gastric glands that had greater HER-2 immunoexpression (p = 0.0001). A statistically significant association (p = 0.007) between the H. Heilmannii infection score and the expression of HER-2 in the lateral membrane of gastric surface cells was observed. HER-2 expression may be increased in feline gastric cells infected by H. Heilmannii and in parietal cells of gastric glands with an increased inflammatory infiltrate.

中文翻译：

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表皮生长因子受体2在家猫感染Heilmannii感染的胃细胞中的免疫表达

这项研究的目的是评估HER-2在非幽门螺杆菌（NHPH）感染的猫的胃细胞中的免疫表达，并探讨其与炎性浸润的关系。从兽医解剖病理学实验室的档案中检索了四十八个石蜡包埋的胃样品，该样品以前通过快速尿素酶测试和细胞学检查显示为NHPH阳性。使用Warthin-Starry染色通过组织病理学证实了NHPH的感染。审查了苏木精-伊红染色的切片，以评估炎症细胞浸润。使用抗幽门螺杆菌抗体和抗HER-2抗体进行免疫组织化学分析。通过PCR进行分子分析以确认幽门螺杆菌的存在。进行统计分析以确定胃样品中H. Heilmannii的存在与HER-2表达之间是否存在关联。通过免疫组织化学，所有样品的NHPH均为阳性，并通过PCR确认为H. Heilmannii。在组织病理学分析中，有56.3％的样本有淋巴细胞和浆细胞浸润，其中52.1％为轻度和4.2％中度。在具有较高HER-2免疫表达的胃腺壁细胞的完整质膜中，胃粘膜的炎性浸润强度明显更高（p = 0.0001）。观察到H. Heilmannii感染评分与胃表面细胞外侧膜中HER-2的表达之间具有统计学意义的关联（p = 0.007）。