Angiotensin II (AngII) regulates pigment synthesis by tyrosinase in melanocytes. To evaluate the association between AngII and coat color formation, we detected the expression distribution of AngII in white and black sheep skins by LC-ESI-MS/MS, western blot, quantitative real-time-PCR (qPCR) and distribution of AngII by immunohistochemistry.Liquid chromatography-electrospray ionization tandem MS (LC-ESI-MS/MS) results showed that AngII was found in white and black skin tissues of sheep. Western blot results verified the LC-ESI-MS/MS results and suggested that AngII was expressed at significantly higher levels in black sheep skins compared with the white sheep skins. Quantitative real time PCR (qRT-PCR) results also revealed that the expression level of AngII mRNA was higher in black sheep skins than that in white sheep skins. Immunohistochemical analysis further demonstrated that AngII protein was localized in the hair bulb and outer root sheath of hair follicle in sheep. In summary, protein and transcripts exhibited the same expression pattern in white and black sheep skins. Furthermore, the expressions of AngII in the hair bulb and outer root sheath of black sheep were stronger than those in white sheep. These results suggested that AngII functions in sheep coat color regulation and offer a novel insight for further investigation on the role of AngII in the coat color formation in sheep.

中文翻译：

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血管紧张素II在与白色和黑色外套颜色相关的绵羊（羊）皮肤中的表达和组织分布分析。

血管紧张素II（AngII）通过黑素细胞中的酪氨酸酶调节色素合成。为了评估AngII与外套颜色形成之间的关联，我们通过LC-ESI-MS / MS，western印迹，定量实时PCR（qPCR）和AngII分布检测了AngII在白羊和黑羊皮肤中的表达分布。液相色谱-电喷雾串联质谱（LC-ESI-MS / MS）结果表明，在绵羊的白色和黑色皮肤组织中发现了AngII。Western印迹结果验证了LC-ESI-MS / MS结果，表明AngII在黑羊皮中的表达水平明显高于白羊皮。实时定量PCR（qRT-PCR）结果还显示，AngII mRNA在黑羊皮中的表达水平高于白羊皮。免疫组织化学分析进一步表明，AngII蛋白位于绵羊毛囊和毛囊外根鞘中。总之，蛋白质和转录本在白羊和黑羊皮中表现出相同的表达模式。此外，黑羊的毛球和外根鞘中的AngII表达强于白羊。这些结果表明，AngII在羊的外套颜色调节中起作用，并为进一步研究AngII在羊的外套颜色形成中的作用提供了新的见解。黑羊的毛鳞茎和外根鞘中的AngII表达强于白羊。这些结果表明，AngII在绵羊的毛色调节中起作用，并为进一步研究AngII在绵羊的毛色形成中的作用提供了新的见解。黑羊的毛鳞茎和外根鞘中的AngII表达强于白羊。这些结果表明，AngII在羊的外套颜色调节中起作用，并为进一步研究AngII在羊的外套颜色形成中的作用提供了新的见解。