Numerous lymphatic anastomosing channels in the lymph nodes are the most demonstrative finding of the rare lesion termed "vascular transformation of lymph node sinuses" (VTS). The mechanism of lymphatic vessel formation in VTS has not been studied. Vessel intussusception contributes to vascular expansion, and intraluminal pillars/posts, interstitial tissue structures or larger pillars (ITSs) and folds are the hallmarks of this process in blood vessels. The aim of this work is to assess whether these hallmarks of intussusception occur in VTS lymphatic vessels, indicating intussusceptive lymphangiogenesis. For this purpose, specimens of five cases of VTS were used for serial histological sections, immunohistochemistry and immunofluorescence in confocal microscopy, which enabled us to demonstrate the 3D image that defines the pillars. The studies showed a) meshworks of lymphatic vessels, which form complex loops, resembling sinuses of lymph nodes, b) presence of intralymphatic pillars, ITSs and folds, with a cover of lymphatic endothelial cells expressing podoplanin and a varying-sized connective core (e.g. collagen), and c) increase of vessel meshwork and linear arrangement, splitting and fusion of ITSs, pillars and folds, with remodelling and segmentation. In conclusion, the development of lymphatic vessel loops, ITSs, pillars and folds with segmentation in VTS supports intussusceptive lymphangiogenesis. This mechanism of intussusception is of interest because it participates in VTS histogenesis, contributes to general knowledge of intussusceptive lymphangiogenesis, which has received less attention than intussusception in blood vessels, and provides a basis for further studies in other lymphatic conditions.

中文翻译：

---

肠套叠性淋巴管生成在淋巴结窦的血管转化中。

淋巴结中的许多淋巴结吻合通道是这种罕见病变的最典型发现，被称为“淋巴结窦的血管转化”（VTS）。尚未研究VTS中淋巴管形成的机制。血管套叠会促进血管扩张，而腔内柱/柱，间质组织结构或较大的柱（ITS）和褶皱是此过程在血管中的标志。这项工作的目的是评估肠套叠的这些标志是否发生在VTS淋巴管中，表明肠套叠性淋巴管生成。为此，共聚焦显微镜将5例VTS的样本用于连续组织切片，免疫组织化学和免疫荧光分析，这使我们能够展示定义支柱的3D图像。研究显示：a）淋巴管网状结构，形成复杂的环，类似于淋巴结的窦道； b）淋巴管内柱，ITS和褶皱的存在，覆盖着表达podoplanin和大小不等的结缔组织的淋巴管内皮细胞（例如胶原），以及c）血管网和线性排列的增加，ITS，柱和褶的分裂和融合，以及重塑和分割。总之，在VTS中分段形成的淋巴管环，ITS，柱和褶皱的发展支持了肠套叠性淋巴管生成。肠套叠的这种机制很有趣，因为它参与了VTS的组织发生，有助于肠套叠性淋巴管生成的常识，与血管套叠相比，肠套叠性淋巴管生成受到的关注较少，