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Effect of medicaments used in endodontic regeneration on the morphological characteristics of bovine radicular dentin: Experimental immature tooth model.
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2019-12-02 , DOI: 10.1002/jemt.23421
Maira C Conte 1 , Cleonice da Silveira Teixeira 1 , Eduardo A Bortoluzzi 1 , Wilson T Felippe 1 , Luciane G P Dos Santos 2 , Mariana T Pandolfo 1 , Patrícia da Agostim Cancelier 1 , Lucas da Fonseca Roberti Garcia 1
Affiliation  

The purpose of this study was to evaluate the effect of different combinations of irrigating solutions and intracanal dressings in the pretreatment of bovine radicular dentin, using an experimental immature tooth model. Eighty healthy bovine teeth, simulated with incomplete rhizogenesis, were randomly distributed according to the protocols of root canal dentin pretreatment for a regenerative endodontic procedure (n = 10): Control (irrigation with distilled water); SH (irrigation with 1.5% Sodium Hypochlorite); EDTA (irrigation with 17% EDTA); SH/EDTA (irrigation with 1.5% SH + 17% EDTA); SH/CH/EDTA (irrigation with 1.5% SH + calcium hydroxide paste +17% EDTA); SH/MTAP/EDTA (irrigation with 1.5% SH + modified triple antibiotic paste + EDTA 17%); SH/TAP/EDTA (irrigation with 1.5% SH + triple antibiotic paste +17% EDTA) and SH/DAP/EDTA (irrigation with 1.5% SH + double antibiotic paste + EDTA 17%). After the completion of the protocol, the demineralization, the exposure of collagen fibers, and the dentin erosion was evaluated under scanning electron microscopy (SEM), by applying a score system (1–3) to classify the observed features. Statistical analysis was performed (Kruskal‐Wallis and Dunn Multiple Comparison tests—p < .05). SH/TAP/EDTA and SH/DAP/EDTA groups presented the highest rates of demineralization in both the coronal and middle thirds of the root (p < .05). In the SH/MTAP/EDTA group, the samples presented moderate demineralization. The samples from the SH/CH/EDTA group presented similar findings to the control group (p < .05). Conventional triple antibiotic (TAP) and double antibiotic (DAP) pastes promoted more pronounced morphological changes on the dentin surface.

中文翻译:

牙髓再生中使用的药物对牛放射状牙本质形态特征的影响:实验性未成熟牙齿模型。

这项研究的目的是使用实验性的未成熟牙齿模型评估冲洗液和管内敷料的不同组合对牛放射状牙本质的预处理效果。80颗健康牛牙齿,与不完全生根模拟,根据根管的协议牙本质预处理再生牙髓过程,随机分布的(Ñ= 10):控制(用蒸馏水灌溉); SH(用1.5%的次氯酸钠灌溉);EDTA(用17%EDTA灌溉);SH / EDTA(用1.5%SH + 17%EDTA灌溉); SH / CH / EDTA(用1.5%SH +氢氧化钙浆+ 17%EDTA灌溉);SH / MTAP / EDTA(用1.5%SH +改良的三重抗生素糊剂+ EDTA 17%灌溉); SH / TAP / EDTA(用1.5%SH +三倍抗生素糊剂+ 17%EDTA灌溉)和SH / DAP / EDTA(用1.5%SH +双重抗生素糊剂+ EDTA 17%灌溉)。方案完成后,通过应用评分系统(1-3)对观察到的特征进行分类,在扫描电子显微镜(SEM)下评估脱矿质,胶原纤维的暴露以及牙本质的侵蚀。进行统计分析(Kruskal-Wallis和Dunn多重比较检验-p <.05)。SH / TAP / EDTA和SH / DAP / EDTA组在冠状冠状和中部三分之一处的脱矿率最高(p  <.05)。在SH / MTAP / EDTA组中,样品呈现中等程度的脱矿质。SH / CH / EDTA组的样品表现出与对照组相似的发现(p  <.05)。常规的三联抗生素(TAP)和双联抗生素(DAP)糊剂可促进牙本质表面的形态变化。
更新日期:2019-12-02
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