SummaryThe aim of this study was to characterize the protein profile of ovarian follicular fluid (FF) of brown brocket deer (Mazama gouazoubira). Five adult females received an ovarian stimulation treatment and the FF was collected by laparoscopy from small/medium (≤3.5 mm) and large (>3.5 mm) follicles. Concentrations of soluble proteins in FF samples were measured and proteins were analyzed by 1-D SDS-PAGE followed by tryptic digestion and tandem mass spectrometry. Data from protein list defined after a Mascot database search were analyzed using the STRAP software tool. For the protein concentration, no significant difference (P > 0.05) was observed between small/medium and large follicles: 49.2 ± 22.8 and 56.7 ± 27.4 μg/μl, respectively. Mass spectrometry analysis identified 13 major proteins, but with no significant difference (P > 0.05) between follicle size class. This study provides insight into elucidating folliculogenesis in brown brocket deer.

中文翻译：

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褐斑鹿卵巢卵泡液的蛋白质谱 (Mazama gouazoubira; Fisher, 1814)

摘要本研究的目的是描述褐斑鹿卵巢卵泡液 (FF) 的蛋白质谱。马扎马古阿祖比拉）。五名成年女性接受了卵巢刺激治疗，并通过腹腔镜从小/中（≤3.5 mm）和大（>3.5 mm）卵泡收集FF。测量 FF 样品中可溶性蛋白质的浓度，并通过 1-D SDS-PAGE、胰蛋白酶消化和串联质谱分析蛋白质。使用 STRAP 软件工具分析 Mascot 数据库搜索后定义的蛋白质列表中的数据。对于蛋白质浓度，没有显着差异（磷> 0.05) 在小/中型和大卵泡之间观察到：分别为 49.2 ± 22.8 和 56.7 ± 27.4 μg/μl。质谱分析鉴定出 13 种主要蛋白质，但没有显着差异（磷> 0.05) 在卵泡大小等级之间。这项研究为阐明褐斑鹿的卵泡发生提供了见解。