Human bocavirus (HBoV) has a global distribution and is associated with respiratory and enteric infections, particularly in the paediatric population. In this study, raw sewage and mussel samples were analysed for the presence of HBoV using nested PCR with primers targeting the VP1/VP2 junction. Amplification and sequencing of the 382 bp region followed by phylogenetic analysis indicated the presence of HBoV 2 in mussel samples and HBoV 3 in sewage samples. This is the first report describing the presence of enteric-associated HBoV in environmental samples from South Africa and in mussel samples from the African continent. The results signify the need for further studies examining the potential risk of foodborne transmission of HBoV and highlight the importance of continued screening to determine the prevalence and epidemiology of HBoV in South Africa.

中文翻译：

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南非首次在人类污水和贻贝中发现人类Bocavirus物种2和3。

人博卡病毒（HBoV）具有全球分布，并与呼吸道和肠道感染有关，尤其是在儿科人群中。在这项研究中，使用嵌套式PCR和靶向VP1 / VP2连接的引物，分析了原始污水和贻贝样品中是否存在HBoV。382 bp区域的扩增和测序以及随后的系统发育分析表明，贻贝样品中存在HBoV 2，污水样品中存在HBoV 3。这是第一份报告，描述了南非环境样品和非洲大陆贻贝样品中存在肠道相关的HBoV。