Fat-mass and obesity-associated protein (Fto) plays important roles in energy metabolism. It also acts as a demethylase and is most abundantly found in the brain. In the present study, we examined the spatial and temporal changes of Fto immunoreactivity after five minutes of transient forebrain ischemia in the hippocampus. In the control group, Fto immunoreactivity was mainly observed in the nucleus of pyramidal cells in the CA1 and CA3 regions as well as the polymorphic layer, granule cell layer, and subgranular zone of the dentate gyrus. Fto immunoreactivity was transiently, but not significantly, increased in the hippocampal CA3 region and the dentate gyrus two days after ischemia compared to mice without ischemia in the sham-operated group. Four days after ischemia, low Fto immunoreactivity was observed in the stratum pyramidale of the CA1 region because of neuronal death, but Fto immunoreactive cells were abundantly detected in the stratum pyramidale of the CA3 region, which is relatively resistant to ischemic damage. Thereafter, Fto immunoreactivity progressively decreased in the hippocampal CA1 and CA3 regions and the dentate gyrus until ten days after ischemia. At this time-point, Fto immunoreactivity was significantly lower in the hippocampal CA1 and CA3 regions and the dentate gyrus compared to that in the sham-operated group. The reduction of Fto immunoreactive structures in the hippocampus may be associated with impairments in Fto-related hippocampal function.

中文翻译：

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沙鼠海马中与缺血相关的脂肪和肥胖相关蛋白表达的变化。

脂肪和肥胖相关蛋白（Fto）在能量代谢中起重要作用。它也起脱甲基酶的作用，在大脑中含量最高。在本研究中，我们检查了海马短暂性前脑缺血五分钟后Fto免疫反应性的时空变化。在对照组中，Fto免疫反应性主要在CA1和CA3区的锥体细胞核以及齿状回的多态性层，颗粒细胞层和亚颗粒区中观察到。与假手术组中没有缺血的小鼠相比，缺血后两天，海马CA3区和齿状回的Fto免疫反应性是短暂的，但没有明显增加。缺血四天后 由于神经元死亡，在CA1区锥体层中观察到低Fto免疫反应性，但在CA3区锥体层中大量检测到Fto免疫反应性细胞，对缺血性损伤具有相对抗性。此后，直到缺血后十天，海马CA1和CA3区域以及齿状回的Fto免疫反应性逐渐降低。在这个时间点上，与假手术组相比，海马CA1和CA3区以及齿状回中的Fto免疫反应性显着降低。海马中Fto免疫反应性结构的减少可能与Fto相关的海马功能受损有关。相对抗缺血性损伤。此后，直到缺血后十天，海马CA1和CA3区域以及齿状回的Fto免疫反应性逐渐降低。在这个时间点上，与假手术组相比，海马CA1和CA3区以及齿状回中的Fto免疫反应性显着降低。海马中Fto免疫反应性结构的减少可能与Fto相关的海马功能受损有关。相对抗缺血性损伤。此后，直到缺血后十天，海马CA1和CA3区域以及齿状回的Fto免疫反应性逐渐降低。在这个时间点上，与假手术组相比，海马CA1和CA3区以及齿状回中的Fto免疫反应性显着降低。海马中Fto免疫反应性结构的减少可能与Fto相关的海马功能受损有关。与假手术组相比，海马CA1和CA3区以及齿状回的Fto免疫反应性明显较低。海马中Fto免疫反应性结构的减少可能与Fto相关的海马功能受损有关。与假手术组相比，海马CA1和CA3区以及齿状回的Fto免疫反应性明显较低。海马中Fto免疫反应性结构的减少可能与Fto相关的海马功能受损有关。