Glutathione peroxidases (GPx), major antioxidant enzymes, secreted by Fasciola spp., are important for the parasite evasion and protection against the host's immune responses. In the present study, a monoclonal antibody (MoAb) against recombinant F. gigantica glutathione peroxidase (rFgGPx) was produced by hybridoma technique using spleen cells from BALB/c mice immunized with rFgGPx. This MoAb (named 7B8) is IgG1 with κ light chains, and it reacted specifically with rFgGPx at a molecular weight 19 kDa as shown by immunoblotting, and reacted with the native FgGPx in the extracts of whole body (WB), metacercariae, newly excysted juveniles (NEJs), 4 week-old juveniles and adult F. gigantica as shown by indirect ELISA. It did not cross react with antigens in WB fractions from other adult trematodes, including Fischoederius cobboldi, Paramphistomum cervi, Setaria labiato-papillosa, Eurytrema pancreaticum, Gastrothylax crumenifer and Gigantocotyle explanatum. By immunolocalization, MoAb against rFgGPx reacted with the native protein in the tegument, vitelline cells, and eggs of adult F. gigantica. In addition, the sera from mice experimentally infected with F. gigantica were tested positive by this indirect sandwich ELISA. This result indicated that FgGPx is an abundantly expressed parasite protein that is secreted into the tegumental antigens (TA), therefore, FgGPx and its MoAb may be used for immunodiagnosis of both early and late fasciolosis gigantica in animals and humans.

中文翻译：

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巨大的Fasciola gigantica谷胱甘肽过氧化物酶单克隆抗体及其对fasciolosis的免疫诊断潜力。

Fasciola spp。分泌的谷胱甘肽过氧化物酶（GPx）是主要的抗氧化酶，对于逃避寄生虫和防止宿主的免疫反应非常重要。在本研究中，通过杂交瘤技术，使用免疫rFgGPx的BALB / c小鼠的脾细胞，通过杂交瘤技术生产了针对重组巨巨谷胱甘肽谷胱甘肽过氧化物酶（rFgGPx）的单克隆抗体（MoAb）。这种MoAb（命名为7B8）是具有κ轻链的IgG1，它通过免疫印迹显示与分子量为19 kDa的rFgGPx特异性反应，并与新近被囊化的全身（WB），尾meta虫的提取物中的天然FgGPx反应。间接ELISA显示，幼体（NEJs），4周龄的幼体和成年巨.。它不会与其他成虫吸虫的WB级分中的抗原发生交叉反应，包括Fischoederius cobboldi，子宫颈副鞭毛虫，唇形狗尾草，番石榴叶，Gastrothylax crumenifer和Gigantocotyle explanatum。通过免疫定位，针对rFgGPx的MoAb与成年F.gantica的外皮，卵黄细胞和卵中的天然蛋白质反应。另外，通过间接夹心ELISA检测出实验性感染巨巨镰刀菌的小鼠血清呈阳性。该结果表明，FgGPx是分泌到外皮抗原（TA）中的大量表达的寄生虫蛋白，因此，FgGPx及其MoAb可用于动物和人中的巨型和晚期fasciolosis的免疫诊断。卵黄细胞和成年大卵eggs的卵。另外，通过间接夹心ELISA检测出实验性感染巨巨镰刀菌的小鼠血清呈阳性。该结果表明，FgGPx是分泌到外皮抗原（TA）中的大量表达的寄生虫蛋白，因此，FgGPx及其MoAb可用于动物和人中的巨型和晚期fasciolosis的免疫诊断。卵黄细胞和成年大卵eggs的卵。另外，通过间接夹心ELISA检测出实验性感染巨巨镰刀菌的小鼠血清呈阳性。该结果表明，FgGPx是分泌到外皮抗原（TA）中的大量表达的寄生虫蛋白，因此，FgGPx及其MoAb可用于动物和人中的巨型和晚期fasciolosis的免疫诊断。