We report the first comparative study of protein expression profiles in tuber sprouts between Katahdin-derived potato cultivars resistant and susceptible to Synchytrium endobioticum. Synchytrium endobioticum causes wart disease in potato (Solanum tuberosum L.) and is considered as the most important quarantine pathogen in almost all countries where potatoes are grown. We performed a comparative analysis of differentially expressed proteins in the tuber sprouts of potato cultivars differing in resistance to pathotype 1(D1) of S. endobioticum using two-dimensional electrophoresis (2-DE) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) approaches. Bulks prepared from two resistant (Calrose and Humalda) and three susceptible (Sebago, Seneca and Wauseon) potato cultivars were studied. When protein profiles were compared between mock- and S. endobioticum-inoculated sprouts, 35 and 63 protein spots, indicating qualitative or quantitative differences, were detected in the resistant and susceptible cultivars, respectively. In turn, 24 proteins associated with resistance to S. endobioticum were revealed by comparison of the resistant and susceptible bulks. These proteins were changed in a constitutive or induced manner and were grouped into four categories: stress and defence, cell structure, protein turnover, and metabolism. Among the 13 proteins classified into the stress and defence group, seven proteins were related to heat-shock proteins (HSPs)/chaperone factors. In addition, four proteins, S-adenosyl-l-homocysteine hydrolase-like, superoxide dismutase [Mn], inactive patatin-3-Kuras 1 and patatin-15, were induced in the resistant bulk; whereas two proteins, patatin-01 and nucleoredoxin 1, showed significant differences in expression between the S. endobioticum-inoculated resistant and susceptible bulks. The detection of such a large number of S. endobioticum-mediated proteins representing the HSP70, HSP60 and HSP20 families suggests their significant role in restricting wart disease in potato tubers.

中文翻译：

---

Synchytrium endobioticum侵染期间抗性和易感马铃薯品种的比较蛋白质组学分析

我们报告了对 Katahdin 衍生的马铃薯品种抗性和易感 Synchytrium endobioticum 块茎芽中蛋白质表达谱的第一次比较研究。Synchytrium endobioticum 会导致马铃薯 (Solanum tuberosum L.) 发生疣病，并且在几乎所有马铃薯种植国被认为是最重要的检疫病原体。我们使用二维电泳 (2-DE) 和液相色谱-串联质谱 (LC-MS /MS) 方法。研究了由两种抗性（Calrose 和 Humalda）和三种易感（Sebago、Seneca 和 Wauseon）马铃薯品种制备的块状马铃薯。当在模拟和 S 之间比较蛋白质谱时。在抗病品种和易感品种中分别检测到内生菌接种的芽，35个和63个蛋白质斑点，表明存在定性或定量差异。反过来，通过比较抗性和易感性块体，揭示了 24 种与内生链球菌抗性相关的蛋白质。这些蛋白质以组成或诱导的方式发生变化，并分为四类：压力和防御、细胞结构、蛋白质周转和新陈代谢。在归类为应激和防御组的 13 种蛋白质中，有 7 种蛋白质与热休克蛋白 (HSP)/伴侣因子有关。此外，四种蛋白质，S-腺苷-l-同型半胱氨酸水解酶样、超氧化物歧化酶[Mn]、无活性的patatin-3-Kuras 1和patatin-15，在抗性体中被诱导；而两种蛋白质，patatin-01 和 nucleoredoxin 1，在 S. endobioticum 接种的抗性和易感群体之间表现出显着差异。检测到如此大量的 S. endobioticum 介导的 HSP70、HSP60 和 HSP20 家族蛋白表明它们在限制马铃薯块茎中的疣病方面发挥着重要作用。