miRNA targets from Citrus sinensis are predicted and validated using degradome data. They show an up-regulation upon infection with CPsV, with a positive correlation between target expression and symptom severity. Sweet orange (Citrus sinensis) may suffer from disease symptoms induced by virus infections, thus resulting in drastic economic losses. Infection of sweet orange plants with two isolates of citrus psorosis virus (CPsV), expressing different symptomatologies, alters the accumulation of a set of endogenous microRNAs (miRNAs). Here, we predicted ten putative targets from four down-regulated miRNAs: three belonging to the CCAAT-binding transcription factor family (CBFAs); an Ethylene-responsive transcription factor (RAP2-7); an Integrase-type DNA-binding superfamily protein (AP2B); Transport inhibitor response 1 (TIR1); GRR1-like protein 1-related (GRR1); Argonaute 2-related (AGO2), Argonaute 7 (AGO7), and a long non-coding RNA (ncRNA). We validated six of them through analysis of leaf degradome data. Expressions of the validated targets increase in infected samples compared to healthy tissue, showing a more striking up-regulation those samples with higher symptom severity. This study contributes to the understanding of the miRNA-mediated regulation of important transcripts in Citrus sinensis through target validation and shed light in the manner a virus can alter host regulatory mechanisms leading to symptom expression.

中文翻译：

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在感染两种不同柑橘银屑病病毒分离株的柑橘植物中，microRNA 靶标的上调与症状严重程度相关

使用降解组数据预测和验证来自 Citrus sinensis 的 miRNA 靶标。它们在感染 CPsV 后表现出上调，目标表达与症状严重程度之间呈正相关。甜橙（Citrus sinensis）可能因病毒感染而出现疾病症状，从而造成巨大的经济损失。用表达不同症状的两种柑橘类银屑病病毒 (CPsV) 分离株感染甜橙植物会改变一组内源性微 RNA (miRNA) 的积累。在这里，我们从四个下调的 miRNA 中预测了十个推定的目标：三个属于 CCAAT 结合转录因子家族 (CBFA)；乙烯反应性转录因子 (RAP2-7)；整合酶型 DNA 结合超家族蛋白 (AP2B)；运输抑制剂反应 1 (TIR1); GRR1 样蛋白 1 相关 (GRR1); Argonaute 2 相关 (AGO2)、Argonaute 7 (AGO7) 和长非编码 RNA (ncRNA)。我们通过对叶片降解组数据的分析验证了其中的六个。与健康组织相比，受感染样本中验证目标的表达增加，表明症状严重程度较高的样本上调更为显着。这项研究有助于通过目标验证了解 miRNA 介导的柑橘重要转录本调控，并阐明病毒可以改变导致症状表达的宿主调控机制的方式。与健康组织相比，受感染样本中验证目标的表达增加，表明症状严重程度较高的样本上调更为显着。这项研究有助于通过目标验证了解 miRNA 介导的柑橘重要转录本调控，并阐明病毒可以改变导致症状表达的宿主调控机制的方式。与健康组织相比，受感染样本中验证目标的表达增加，表明症状严重程度较高的样本上调更为显着。这项研究有助于通过目标验证了解 miRNA 介导的柑橘重要转录本调控，并阐明病毒可以改变导致症状表达的宿主调控机制的方式。