Microorganisms produce extracellular compounds that affect the final product quality in fermentation processes. Selection of overproducing mutants requires coupling of the extracellular product to the producer genotype, which can be achieved by single-cell compartmentalization. Emulsions contain up to billions of microdroplets/mL which significantly increases the screening throughput compared to microtiter-plate-based selections. Factors affecting the success of screening in microdroplets include the nature of the producing organism (robust, no invasive growth), the product (not soluble in oil) and the product assay (preferably fluorescence based). Together these factors determine the required microdroplet production technique and sorting set-up. Because microdroplets allow relatively inexpensive ultrahigh-throughput screening, they are likely to become a standard tool in the strain selection toolbox of the fermentation industry.

中文翻译：

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微滴筛选和选择可改善细胞外化合物的微生物产生。

微生物产生影响发酵过程中最终产品质量的细胞外化合物。选择过量生产的突变体需要将细胞外产物与生产者基因型偶联，这可以通过单细胞区室化来实现。乳剂含有多达数十亿微滴/ mL，与基于微量滴定板的选择相比，显着提高了筛选通量。影响微滴筛选成功的因素包括生产生物的性质（稳健，无侵袭性生长），产物（不溶于油）和产物测定法（优选基于荧光）。这些因素共同决定了所需的微滴生产技术和分选设置。由于微滴允许相对便宜的超高通量筛选，