In this study, the circadian rhythm of IgG2 and IgA specific antibodies in serum and saliva samples of 6 dogs experimentally infected with Leishmania infantum was assessed. Sampling was performed at 8.00, 12.00, 16.00, 20.00, and 00.00 h on two consecutive days. Anti-Leishmania antibody levels in serum were expressed without any correction, whereas in saliva were shown in different ways: without any correction, adjusted by protein concentration and corrected by the salivary flow rate. No significant differences in anti-Leishmania IgG2 antibody levels in serum and saliva samples with or without correction were found. Significant differences were found when anti-Leishmania IgA levels were corrected by the salivary flow rate. In addition, a greater intra-individual variation of antibody levels was observed in saliva than in serum. However, this variation did not modify the serological status of the dogs. Therefore, it could be concluded that there is no circadian rhythm in serum and saliva samples and sampling can be performed at any time of the day.

中文翻译：

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评估临床利什曼病犬的血清和唾液中抗利什曼原虫IgG2和IgA抗体的昼夜节律。

在这项研究中，评估了实验性感染婴儿利什曼原虫的6只狗的血清和唾液样本中IgG2和IgA特异性抗体的昼夜节律。在连续两天的8.00、12.00、16.00、20.00和00.00 h进行采样。血清中的抗利什曼原虫抗体水平未经任何校正即可表达，而唾液中的抗体表达则有不同的显示方式：未经任何校正，可通过蛋白质浓度进行调整并通过唾液流速进行调整。在校正或未校正的血清和唾液样品中，抗利什曼原虫IgG2抗体水平均无显着差异。通过唾液流速校正抗利什曼原虫IgA水平时发现了显着差异。另外，在唾液中观察到的抗体水平的个体内变化大于血清中的个体水平。然而，这种变化没有改变狗的血清状态。因此，可以得出结论，血清和唾液样本中没有昼夜节律，可以在一天中的任何时间进行采样。