Glycan-mediated interactions are essential in many biological processes and regulate a wide variety of cellular functions. However, characterizing these interactions is difficult because glycan biosynthesis is not template driven and because carbohydrate recognition events are usually of low affinity and transient. Photocrosslinking carbohydrate probes can form a covalent bond with molecules in close proximity on UV irradiation and are capable of capturing interactions between glycans and glycan-binding proteins in situ. Because of these advantages, multiple photocrosslinking carbohydrate probes have been designed and applied to study the biological functions of glycans. This review will discuss recent advances in the development of novel photocrosslinking functional groups and the design of photocrosslinking probes to detect interactions mediated by glycolipids, peptidoglycan, and multivalent carbohydrate ligands. These probes have demonstrated the potential to address some of the major challenges in the study of glycan-mediated interactions in both model systems and in more complex biological settings.

中文翻译：

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用于捕获碳水化合物相互作用的光交联探针。

聚糖介导的相互作用在许多生物学过程中都是必不可少的，并调节多种细胞功能。然而，表征这些相互作用是困难的，因为聚糖的生物合成不是模板驱动的，并且因为碳水化合物的识别事件通常是低亲和力的和短暂的。光交联的碳水化合物探针可以在紫外线照射下与紧邻的分子形成共价键，并且能够原位捕获聚糖和聚糖结合蛋白之间的相互作用。由于这些优点，已经设计了多种光交联碳水化合物探针并将其用于研究聚糖的生物学功能。这篇综述将讨论新型光交联官能团的发展以及检测由糖脂，肽聚糖和多价碳水化合物配体介导的相互作用的光交联探针的设计的最新进展。这些探针已证明有潜力解决模型系统和更复杂的生物学环境中聚糖介导的相互作用研究中的一些主要挑战。