Proteases have emerged as a promising class of enzymes to build post-translationally regulated signalling functions in diverse organisms and cell types ranging from simple prokaryotes to higher eukaryotes and in reconstituted systems in vitro. An expanding repertoire of proteases can now be readily configured to build tailored sensors, switches and transducers, and is increasingly facilitating the construction of complex sensory systems for a variety of biotechnological and biomedical applications. This is complemented by an increasing understanding of the fundamental design principles underlying biological signal processing at both protein-level and circuit-level that is now actively probed through synthesis. This review thus aims to summarize and analyse the most promising conceptual and experimental approaches that can be applied to build artificial signalling functions with proteases while highlighting advances, drawbacks and limitations.

中文翻译：

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利用蛋白酶工程化人工信号传导功能。

蛋白酶已成为一种有前途的酶，可在多种生物和细胞类型（从简单的原核生物到高级的真核生物）以及体外重组系统中建立翻译后调节的信号传导功能。现在可以轻松配置不断扩展的蛋白酶库，以构建量身定制的传感器，开关和换能器，并且正日益促进为各种生物技术和生物医学应用构建复杂的传感系统。对此的补充是，对蛋白质水平和电路水平的生物信号处理基础的基本设计原理有了越来越多的了解，现在已经通过合成积极地对其进行了探索。