Benjaminiella poitrasii, a zygomycete, shows glucose- and temperature-dependent yeast (Y)-hypha (H) dimorphic transition. Earlier, we reported the biochemical correlation of relative proportion of NAD- and NADP-glutamate dehydrogenases (GDHs) with Y-H transition. Further, we observed the presence of one NAD-GDH and two form-specific NADP-GDH isoenzymes in B. poitrasii. However, molecular studies are necessary to elucidate the explicit role of GDHs in regulating Y-H reversible transition. Here, we report the isolation and characterization of one NAD (BpNADGDH, 2.643 kb) and two separate genes, BpNADPGDH I (Y-form specific, 1.365 kb) and BpNADPGDH II (H-form specific, 1.368 kb) coding for NADP-GDH isoenzymes in B. poitrasii. The transcriptional profiling during Y-H transition showed higher BpNADPGDH I expression in Y cells while expression of BpNADPGDH II was higher in H cells. Moreover, the yeast-form monomorphic mutant (Y-5) did not show BpNADPGDH II expression under normal dimorphism triggering conditions. Transformation with H-form specific BpNADPGDH II induced the germ tube formation in Y-5, which confirmed the cause-effect relationship between BpNADPGDH genes and morphological outcome in B. poitrasii. Interestingly, expression of H-form specific BpNADPGDH II also induced germ tube formation in human pathogenic, non-dimorphic yeast Candida glabrata, which further corroborated our findings.

中文翻译：

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NAD-和NADP-谷氨酸脱氢酶的分子研究破译了合子贝氏迷你酵母中的菌丝-菌丝二态性难题。

合子体Benjaminiella poitrasii显示了葡萄糖和温度依赖性酵母（Y）-菌丝（H）的双态转变。此前，我们报道了NAD和NADP谷氨酸脱氢酶（GDHs）与YH过渡的相对比例的生化相关性。此外，我们观察到在布氏芽孢杆菌中存在一种NAD-GDH和两种形式特异性NADP-GDH同工酶。但是，有必要进行分子研究以阐明GDH在调节YH可逆转变中的明确作用。在这里，我们报告的一个NAD（BpNADGDH，2.643 kb）和两个单独的基因，BpNADPGDH I（Y型特异性，1.365 kb）和BpNADPGDH II（H型特异性，1.368 kb）的编码和分离描述了NADP-GDH。芽孢杆菌中的同工酶。YH过渡期间的转录谱显示在Y细胞中较高的BpNADPGDH I表达，而在H细胞中较高的BpNADPGDH II表达。此外，酵母形式的单态突变体（Y-5）在正常双态触发条件下未显示BpNADPGDH II表达。H型特异性BpNADPGDH II的转化诱导了Y-5胚芽的形成，这证实了BpNADPGDH基因与芽孢杆菌形态结果之间的因果关系。有趣的是，H型特异性BpNADPGDH II的表达也诱导了人类致病性，非二态性光滑念珠菌念珠菌的形成，这进一步证实了我们的发现。H型特异性BpNADPGDH II的转化诱导了Y-5胚芽的形成，这证实了BpNADPGDH基因与芽孢杆菌形态结果之间的因果关系。有趣的是，H型特异性BpNADPGDH II的表达也诱导了人类致病性，非二态性光滑念珠菌念珠菌的胚管形成，这进一步证实了我们的发现。H型特异性BpNADPGDH II的转化诱导了Y-5胚芽的形成，这证实了BpNADPGDH基因与芽孢杆菌形态结果之间的因果关系。有趣的是，H型特异性BpNADPGDH II的表达也诱导了人类致病性，非二态性光滑念珠菌念珠菌的胚管形成，这进一步证实了我们的发现。