The duplication of chromosomal DNA is a key cell cycle event that involves the controlled, bidirectional assembly of the replicative machinery. In a tightly regulated, multi-step reaction, replicative helicases and other components of the DNA synthesis apparatus are recruited to replication start sites. Although the molecular approaches for assembling this machinery vary between the different domains of life, a common theme revolves around the use of ATP-dependent initiation factors to recognize and remodel origins and to load replicative helicases in a bidirectional manner onto DNA. This review summarizes recent advances in understanding the mechanisms of replication initiation in eukaryotes, focusing on how the replicative helicase is loaded in this system.

中文翻译：

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复制源许可的机制：结构角度。

染色体DNA的复制是一个关键的细胞周期事件，涉及复制机器的受控双向组装。在严格调节的多步骤反应中，将复制解旋酶和DNA合成装置的其他组件募集到复制起始位点。尽管组装这种机器的分子方法在生活的不同领域之间有所不同，但一个共同的主题是围绕使用ATP依赖的起始因子来识别和重塑来源并将双向解旋酶双向加载到DNA上。这篇综述总结了在了解真核生物中复制起始机制方面的最新进展，重点是在该系统中如何加载复制解旋酶。