Listeria monocytogenes is a foodborne pathogen. The recent alert for L. monocytogenes in vegetables from Argentina warns about the importance of reinforcing its isolation, characterization and subtyping in food, clinical and environmental samples. The aim of the present study was to compare the discriminatory power of enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR), automated ribotyping and pulsed-field gel electrophoresis (PFGE) to subtype strains of L. monocytogenes isolated from Argentine meat and environmental samples. Simpson's Diversity Index (DI) was calculated on the basis of based on the dendrograms obtained in the by cluster analysis, showing the following discriminatory power: ApaI-PFGE (0.980), AscI-PFGE (0.966), ribotyping (0.912), ERIC-PCR (0.886). The ID values between ApaI- and AscI-PFGE and between ribotyping and ERIC-PCR were not significantly different. Of the three techniques evaluated, PFGE showed the highest discriminatory power. However, the subtyping techniques should be accompanied by effective food monitoring strategies and reliable clinical and epidemiological studies.

单核细胞增生李斯特菌是食源性病原体。最近来自阿根廷蔬菜中单核细胞增生李斯特氏菌的警报警告，加强其在食品，临床和环境样品中的分离，鉴定和亚型化的重要性。本研究的目的是比较肠细菌重复基因间共有聚合酶链反应（ERIC-PCR），自动核糖分型和脉冲场凝胶电泳（PFGE）对从阿根廷肉类和环境中分离出的单核细胞增生李斯特菌亚型的区分能力样品。辛普森多样性指数（DI）是基于，通过基于聚类分析获得的树状图计算得出的，显示出以下歧视能力：Apa I-PFGE（0.980），Asc I-PFGE（0.966），核糖分型（0.912），ERIC-PCR（0.886）。Apa I-和Asc I-PFGE之间的ID值以及核糖分型和ERIC-PCR之间的ID值没有显着差异。在评估的三种技术中，PFGE表现出最高的辨别力。但是，分型技术应伴有有效的食品监测策略以及可靠的临床和流行病学研究。