Aflatoxin is a carcinogenic secondary metabolite produced mainly by Aspergillus flavus and Aspergillus parasiticus, which can seriously endanger the health of humans and animals. Oxidative stress is a common defense response, and it is known that reactive oxygen species (ROS) can induce the synthesis of a series of secondary metabolites, including aflatoxin. By using mutants lacking the afap 1 gene, the role of afap 1 gene in oxidative stress and aflatoxin synthesis was assessed. The growth of the mutant strains was significantly inhibited by the increase in the concentration of H₂O₂, inhibition was complete at 40 mmol/l. However, in the quantitative analysis by HPLC, the concentration of AFB1 increased with the increased H₂O₂ until 10 mmol/l. Following an analysis based on the information provided by the NCBI BLAST analysis, it was assumed that Afap1, a basic leucine zipper (bZIP) transcription factor, was associated with the oxidative stress in this fungus. Treatment with 5 mmol/l H₂O₂ completely inhibited the growth of the mutant strains in afap 1 but did not affect the growth of the CA14PTs strain (non-mutant strain). In addition, the concentration of AFB₁ in the mutant strains was approximately ¼ of that observed in the CA14PTs strain. These results suggested that Afap1 plays a key role in the regulation of oxidative stress and aflatoxin production in A. flavus.

黄曲霉毒素是一种主要由黄曲霉和寄生曲霉产生的致癌次生代谢产物，可严重危害人类和动物的健康。氧化应激是一种常见的防御反应，众所周知，活性氧（ROS）可以诱导一系列次级代谢产物的合成，包括黄曲霉毒素。通过使用缺少afap 1基因的突变体，评估了afap 1基因在氧化应激和黄曲霉毒素合成中的作用。H ₂ O ₂浓度的增加显着抑制了突变菌株的生长。抑制作用在40 mmol / l时完成。然而，在通过HPLC的定量分析中，AFB1的浓度随着H ₂ O _2的增加而增加，直到10mmol / l。根据NCBI BLAST分析提供的信息进行分析后，假定碱性亮氨酸拉链（bZIP）转录因子Afap1与这种真菌的氧化应激有关。用5 mmol / l H ₂ O _2处理完全抑制了afap 1中突变菌株的生长，但不影响CA14PTs菌株（非突变菌株）的生长。此外，AFB ₁的浓度在突变株中的大约是在CA14PTs株中观察到的的1/4。这些结果表明，Afap1起着氧化应激和黄曲霉毒素的产生在调控关键作用黄曲霉。