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Identification of an Argentinean isolate of Spodoptera frugiperda granulovirus.
Revista Argentina de Microbiología ( IF 1.8 ) Pub Date : 2019-02-20 , DOI: 10.1016/j.ram.2018.10.003
Matías Luis Pidre 1 , Karina Belén Sabalette 1 , Víctor Romanowski 1 , María Leticia Ferrelli 1
Affiliation  

The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is an important maize pest. Due to the environmental impact and emergence of resistance caused by chemical pesticides and transgenic events, the use of baculoviruses becomes a safe and useful alternative for its control in integrated pest management strategies. Here we report the identification of a novel isolate of a granulovirus of S. frugiperda native to the central region of Argentina, named SfGV ARG. We observed that larvae infected with SfGV ARG showed a yellowish coloration, swollen body and, in some cases, severe lesions in the last abdominal segments. We confirmed the identity of the isolate by sequencing fragments of the lef-8, lef-9 and granulin genes and by calculating evolutionary distances using the Kimura-2-Parameter model. SfGV ARG DNA restriction pattern allowed to estimate a genome of at least 135 kb.



中文翻译:

鉴定了贪食贪夜蛾颗粒病毒阿根廷分离株。

秋天的夜蛾,Spodoptera frugiperda(JE Smith)(鳞翅目:夜蛾科)是一种重要的玉米害虫。由于对环境的影响以及化学农药和转基因事件引起的抗药性的出现,杆状病毒的使用已成为在虫害综合治理策略中控制其的安全和有用的替代方法。在这里,我们报告鉴定的一种新分离株的S. frugiperda颗粒病毒,原产于阿根廷中部地区,名为SfGV ARG。我们观察到,感染了SfGV ARG的幼虫呈淡黄色,身体肿胀,在某些情况下,在最后一个腹部节段出现严重病变。我们通过对lef-8lef-9颗粒蛋白基因,并使用Kimura-2-Parameter模型计算进化距离。SfGV ARG DNA限制性图谱可估计至少135 kb的基因组。

更新日期:2019-02-20
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