The combined action of shikonin and silver nanoparticles (AgNPs) for apoptosis in human cancer cells has not been elucidated. Hence, we investigated the synergistic combinatorial effect of shikonin and AgNPs in human lung cancer cells. Shikonin was used as a reducing and capping agent for AgNPs synthesis as a green method avoiding the hazards of chemical methods. Radiolabeling of Shikonin-AgNPs with radioactive iodine forming [131 I]I-Shikonin-AgNPs was carried out to enable the intracellular tracking of nanoparticles. The antitumor effect of a combined treatment (shikonin-AgNPs) was evaluated using tissue culture assay. The 50% inhibitory concentration (IC50 ) of SHK-AgNPs on A549 cells after 24h determined by an MTT assay is 2.4 ± 0.11μg/mL. As a deduction this study revealed that the combination of shikonin and AgNPs treatment significantly inhibited cell viability and proliferation of A549 cells (human lung carcinoma cell line) with a great potential than the monotherapy.

中文翻译：

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Shikonin-Silver Nanoparticles 的协同细胞毒性作为肺癌的机会

紫草素和银纳米粒子 (AgNPs) 对人类癌细胞凋亡的联合作用尚未阐明。因此，我们研究了紫草素和 AgNPs 在人肺癌细胞中的协同组合作用。Shikonin 被用作 AgNPs 合成的还原剂和封端剂，作为一种绿色方法，避免了化学方法的危害。进行紫草素-AgNPs 与放射性碘形成[ 131 I] I-紫草素-AgNPs 的放射性标记，以实现纳米颗粒的细胞内追踪。使用组织培养测定评估联合治疗（紫草素-AgNPs）的抗肿瘤作用。通过MTT测定法测定24小时后SHK-AgNPs对A549细胞的50%抑制浓度(IC50)为2.4±0.11μg/mL。