Liver metastasis is the most common form of metastatic colorectal cancers during the course of the disease. The global change in protein abundance in liver metastatic colorectal cancers and its role in metastasis establishment have not been comprehensively analyzed. In the present study, fresh-frozen tissue samples including normal colon/localized/liver metastatic CRCs from each recruited patient were analyzed by quantitative proteomics using a multiplexed TMT labeling strategy. Around 5000 protein groups were quantified from all samples. The proteomic profile of localized/metastatic CRCs varied greatly from that of normal colon tissues; differential proteins were mainly from extracellular regions and participate in immune activities, which is crucial for the chronic inflammation signaling pathways in the tumor microenvironment. Further statistical analysis revealed 47 proteins exhibiting statistical significance between localized and metastatic CRCs, of which FILI1P1 and PLG were identified for the first time in proteomic data, which were highly associated with liver metastasis in CRCs.

中文翻译：

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基于质谱的蛋白质组学特征的深度表征揭示了与肝转移性大肠癌相关的新型特征蛋白。

肝转移是在疾病过程中转移性结直肠癌的最常见形式。肝转移性大肠癌中蛋白质丰度的总体变化及其在转移建立中的作用尚未得到全面分析。在本研究中，使用多重TMT标记策略，通过定量蛋白质组学分析了来自每个招募患者的包括正常结肠/局部/肝脏转移性CRC的新鲜冷冻组织样品。从所有样品中量化了大约5000种蛋白质组。局部/转移性CRC的蛋白质组学概况与正常结肠组织的蛋白质组概况差异很大。差异蛋白主要来自细胞外区域，并参与免疫活动，这对于肿瘤微环境中的慢性炎症信号传导通路至关重要。