Nucleosome assembly protein 1-like (Nap1l) family plays numerous biological roles including nucleosome assembly, transcriptional regulation, and cell cycle progression. However, the tissue specific in vivo functions of the Nap1l family members remain largely unknown. In this study, we finished the complete expression patterns of nap1l1 and nap1l4a in zebrafish embryos by whole-mount in situ hybridization. We observed maternal existence of nap1l1 transcript and that its zygotic expression is abundant and not spatially restricted at 6 somite stage, while nap1l4a mRNA is not detectable until 6 somite stage when it is weakly transcribed throughout the embryo. At 24 h post-fertilization (hpf), nap1l1 is predominantly expressed in the central nervous system, neural tube, ventral mesoderm, branchial arches, and pectoral fins, while nap1l4a mRNA is throughout the embryo, enriched in the eyes, tectum, and myotomes. As the embryo develops, nap1l1 expression maintains throughout the head, with gradually enriched in the tectum, olfactory vesicle, lens, optic cups, heart, branchial arches, pectoral fins, axial vasculature, pronephros, and lateral line neuromasts, whereas nap1l4a expression is weak in the tectum, branchial arches, and pectoral fins. Overall, these expression analyses provide a valuable basis for the functional study of nap1l family in zebrafish development.

核小体装配蛋白1样（Nap11）家族起着许多生物学作用，包括核小体装配，转录调控和细胞周期进程。然而，Nap11家族成员的组织特异性体内功能仍然未知。在这项研究中，我们通过整装原位杂交完成了nap1l1和nap1l4a在斑马鱼胚胎中的完整表达模式。我们观察到母亲的nap1l1转录本的存在，其合子表达丰富，并且在6个体节阶段不受空间限制，而nap1l4a直到6个体节阶段，在整个胚胎中都难以转录时，才能检测到mRNA。受精后24小时（hpf），nap1l1主要在中枢神经系统，神经管，腹膜中胚层，branch弓和胸鳍中表达，而nap1l4a mRNA遍及整个胚胎，在眼睛，组织和肌组织中富集。 。随着胚胎的发育，nap1l1的表达在整个头部保持不变，并逐渐丰富了顶盖，嗅觉囊泡，晶状体，视杯，心脏，小arch弓，胸鳍，轴向脉管系统，前肾和侧线神经质，而nap1l4a在顶盖，branch弓和胸鳍中表达较弱。总的来说，这些表达分析为napll家族在斑马鱼发育中的功能研究提供了有价值的基础。