Protein purification is essential in the study of protein structure and function, and the development of novel therapeutics. Many studies require purifying multiple proteins at once, increasing the demand for improved purification methods. We hypothesized that multiple chromatography columns could be interfaced with a multi-well collection plate for rapid and convenient protein purification without the need of expensive instrumentation. As such, we developed a multi-column plate adapter (MCPA), which provides an economical yet versatile and time efficient, high-throughput protein purification system. The MCPA system simultaneously purified milligrams of different proteins under gravity or under vacuum for faster purification. The MCPA handles up to twenty-four 12 mL columns and multiple MCPA's in sequence allow milligram-scale purification of 96 different samples with relative ease. We also used the MCPA system for large scale affinity purification of four proteins, providing sufficient yields and purity for protein crystallization and biophysical characterization. The MCPA system is ideal for optimizing resin type and volume or any other purification parameter by customizing individual columns during the same purification. The high-throughput and versatile nature of this system should prove to be useful in obtaining adequate amounts of protein for subsequent analyses in any laboratory setting.

中文翻译：

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多柱板接头提供了经济且通用的高通量蛋白质纯化系统。

蛋白质纯化对于研究蛋白质的结构和功能以及开发新的疗法至关重要。许多研究要求一次纯化多种蛋白质，从而增加了对改良纯化方法的需求。我们假设多个色谱柱可以与多孔收集板连接，以快速，方便地纯化蛋白质，而无需昂贵的仪器。因此，我们开发了一种多柱板适配器（MCPA），它提供了一种经济而又通用且省时，高效的高通量蛋白质纯化系统。MCPA系统同时在重力或真空下纯化了毫克的不同蛋白质，以加快纯化速度。MCPA可处理多达24个12 mL色谱柱和多个MCPA' 顺序使用s可相对轻松地对96个不同样品进行毫克级纯化。我们还使用MCPA系统对四种蛋白质进行大规模亲和纯化，从而为蛋白质结晶和生物物理表征提供了足够的产量和纯度。MCPA系统是通过在同一纯化过程中定制各个色谱柱来优化树脂类型和体积或任何其他纯化参数的理想选择。该系统的高通量和通用性质应被证明可用于获得足够的蛋白质量，以便在任何实验室环境中进行后续分析。MCPA系统是通过在同一纯化过程中定制各个色谱柱来优化树脂类型和体积或任何其他纯化参数的理想选择。该系统的高通量和通用性质应被证明可用于获得足够的蛋白质量，以便在任何实验室环境中进行后续分析。MCPA系统是通过在同一纯化过程中定制各个色谱柱来优化树脂类型和体积或任何其他纯化参数的理想选择。该系统的高通量和通用性质应被证明可用于获得足够的蛋白质量，以便在任何实验室环境中进行后续分析。