BACKGROUND Porites astreoides is a ubiquitous species of coral on modern Caribbean reefs that is resistant to increasing temperatures, overfishing, and other anthropogenic impacts that have threatened most other coral species. We assembled and annotated a transcriptome from this coral using Illumina sequences from three different developmental stages collected over several years: free-swimming larvae, newly settled larvae, and adults (>10 cm in diameter). This resource will aid understanding of coral calcification, larval settlement, and host-symbiont interactions. FINDINGS A de novo transcriptome for the P. astreoides holobiont (coral plus algal symbiont) was assembled using 594 Mbp of raw Illumina sequencing data generated from five age-specific cDNA libraries. The new transcriptome consists of 867 255 transcript elements with an average length of 685 bases. The isolated P. astreoides assembly consists of 129 718 transcript elements with an average length of 811 bases, and the isolated Symbiodinium sp. assembly had 186 177 transcript elements with an average length of 1105 bases. CONCLUSIONS This contribution to coral transcriptome data provides a valuable resource for researchers studying the ontogeny of gene expression patterns within both the coral and its dinoflagellate symbiont.

中文翻译：

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加勒比石珊瑚 Porites astreoides 三个发育阶段的转录组。


背景滨滨珊瑚是现代加勒比珊瑚礁上普遍存在的珊瑚物种，它能够抵抗温度升高、过度捕捞和威胁大多数其他珊瑚物种的其他人为影响。我们使用多年来收集的三个不同发育阶段的 Illumina 序列组装并注释了该珊瑚的转录组：自由游动的幼虫、新定居的幼虫和成虫（直径>10 cm）。该资源将有助于了解珊瑚钙化、幼虫定居和宿主-共生体相互作用。结果 使用从五个年龄特异性 cDNA 文库生成的 594 Mbp 原始 Illumina 测序数据组装了 P. astreoides holobiont（珊瑚加藻类共生体）的从头转录组。新的转录组由867 255个转录元件组成，平均长度为685个碱基。分离的 P. astreoides 组装体由 129 718 个转录元件组成，平均长度为 811 个碱基，而分离的 Symbiodinium sp. 则由 129 718 个转录元件组成，平均长度为 811 个碱基。组装有 186 177 个转录元件，平均长度为 1105 个碱基。结论对珊瑚转录组数据的贡献为研究珊瑚及其甲藻共生体基因表达模式的个体发育的研究人员提供了宝贵的资源。