To elucidate the role of POU2F2 (POU class 2 homeobox 2) in fracture healing, 30 rats with femoral fracture were randomly grouped into three groups: FF group, LV-POU2F2 group and LV-scramble group. Rats were injected with PBS, lentivirus expressing POU2F2 or scramble lentivirus once a week for 4 weeks. Results showed that overexpressing of POU2F2 promoted fracture healing and callus growth. Besides, overexpressing of POU2F2 promoted protein and mRNA expression of Col10a1, Runx2, Osterix, and Osteocalcin. High Mobility Group AT-hook 1 (HMGA1) is a non-histone protein participating in chromatin remodeling of cells. Western blotting manifested HMGA1/Wnt/β-catenin pathway was activated in POU2F2 group. Moreover, in-vitro study of hMSCs cells supported the above data. In conclusion, POU2F2 promotes fracture healing via activating the HMGA1/Wnt/β-catenin signaling pathway.

中文翻译：

---

POU2F2的过表达通过调节HMGA1 / Wnt /β-catenin信号通路来促进骨折愈合。

为了阐明POU2F2（POU 2类同源异型盒2）在骨折愈合中的作用，将30例股骨骨折大鼠随机分为三组：FF组，LV-POU2F2组和LV争夺组。每周一次给大鼠注射PBS，表达POU2F2的慢病毒或加扰慢病毒，持续4周。结果表明，POU2F2的过表达促进骨折愈合和愈伤组织生长。此外，POU2F2的过表达促进了Col10a1，Runx2，Osterix和Osteocalcin的蛋白质和mRNA表达。高迁移率族AT钩1（HMGA1）是一种非组蛋白，参与细胞的染色质重塑。Western blotting结果显示POU2F2组HMGA1 / Wnt /β-catenin途径被激活。此外，hMSCs细胞的体外研究支持上述数据。结论，