In the developing central nervous system, the terminal differentiation of oligodendrocytes (OLs) is regulated by both extrinsic and intrinsic factors. Recent studies have suggested that the Notch-Hes signaling pathway influences the maturation of oligodendrocytes in culture and during development. However, the specific Notch receptors and their downstream effectors Hes genes that are involved in oligodendrocyte maturation have not been investigated systematically. In this study, we showed that Notch1 and Notch3 are expressed in oligodendrocyte precursor cells (OPCs) during gliogenesis, and Hes5 is the major Notch downstream transcription factor that is transiently expressed in OPCs. Overexpression of Notch intracellular domain (NICD) and Hes5 proteins in embryonic chicken spinal cord suppressed both the endogenous and Sox10-induced Mbp gene expression. Unexpectedly, overexpression of NICD/Hes5 did not inhibit Sox10 induction of Olig2 expression and Myrf induced Mbp expression, suggesting the differential inhibitory effects of NICD/Hes5 signaling on Sox10 activation of myelin-related genes and early progenitor genes.

中文翻译：

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Notch-Hes途径对Sox10功能的差异抑制。

在发育中的中枢神经系统中，少突胶质细胞（OLs）的终末分化受外在和内在因素调节。最近的研究表明，Notch-Hes信号通路会影响培养和发育过程中少突胶质细胞的成熟。但是，尚未系统地研究参与少突胶质细胞成熟的特定Notch受体及其下游效应子Hes基因。在这项研究中，我们表明Notch1和Notch3在胶质细胞生成过程中在少突胶质前体细胞（OPC）中表达，而Hes5是在OPC中瞬时表达的主要Notch下游转录因子。胚胎鸡脊髓中Notch细胞内结构域（NICD）和Hes5蛋白的过表达抑制了内源性和Sox10诱导的Mbp基因表达。出乎意料的是，NICD / Hes5的过表达不会抑制Sox10诱导Olig2表达和Myrf诱导的Mbp表达，这表明NICD / Hes5信号传导对髓鞘相关基因和早期祖细胞基因的Sox10活化具有不同的抑制作用。