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Complete genome sequence of a novel polerovirus in Ornithogalum thyrsoides from South Africa.
Archives of Virology ( IF 2.5 ) Pub Date : 2019-11-28 , DOI: 10.1007/s00705-019-04472-x
I Mostert 1 , M Visser 1 , I Gazendam 2 , M Cloete 2 , J T Burger 1 , H J Maree 1, 3
Affiliation  

Ornithogalum thyrsoides, commonly known as chincherinchee, is an indigenous ornamental plant widely cultivated in South Africa. It is commercially valued as a flowering pot plant and for the production of cut flowers. Virus infections resulting in the development of severe necrotic mosaic symptoms threaten the success of commercial cultivation. The virome of an O. thyrsoides plant displaying necrotic mosaic symptoms was determined using high-throughput sequencing (HTS). In this plant, ornithogalum mosaic virus and ornithogalum virus 3 were identified, as well as a previously unknown virus. The full genome sequence of this virus was confirmed by Sanger sequencing using overlapping amplicons combined with rapid amplification of cDNA ends (RACE). Based on genome organisation and phylogenetic analysis, this novel virus can be classified as a polerovirus.

中文翻译:

来自南非的虎眼百里香中新的杆状病毒的完整基因组序列。

虎眼万年青,俗称chincherinchee,是一种在南非广泛种植的本土观赏植物。它在商业上被视为花盆植物和切花生产的价值。导致严重坏死性花叶病症状发展的病毒感染威胁到商业化种植的成功。使用高通量测序(HTS)确定显示坏死花叶症状的甲状腺球菌植物的病毒。在该植物中,鉴定出了虎眼万年青花叶病毒和虎眼万年青病毒3,以及以前未知的病毒。通过重叠扩增子与cDNA末端的快速扩增(RACE)的Sanger测序,证实了该病毒的完整基因组序列。基于基因组组织和系统发育分析,
更新日期:2020-01-31
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