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Determination of antimicrobial susceptibility and biofilm production in Staphylococcus aureus isolated from white coats of health university students.
Annals of Clinical Microbiology and Antimicrobials ( IF 4.6 ) Pub Date : 2019-11-28 , DOI: 10.1186/s12941-019-0337-6
Isabela Rotta Batista 1 , Amanda Caroline Lima Prates 1 , Bruna de Souza Santos 1 , Josimara Cristina Carvalho Araújo 1 , Yan Christian de Oliveira Bonfim 1 , Marcus Vinícius Pimenta Rodrigues 1 , Glilciane Morceli 1 , Jossimara Polettini 1 , Andressa Cortes Cavalleri 1 , Lizziane Kretli Winkelstroter 1 , Valéria Cataneli Pereira 1
Affiliation  

This study aimed at detecting Staphylococcus aureus from white coats of college students and characterizing antimicrobial susceptibility and biofilm production. Bacterial samples (n = 300) were obtained from white coats of 100 college students from August 2015 to March 2017 S. aureus was isolated and it´s resistance profile was assessed by antimicrobial disk-diffusion technique, screening for methicillin-resistant Staphylococcus aureus (MRSA), detection of mecA gene by PCR, and determination of staphylococcal cassette chromosome mec (SCCmec) by multiplex PCR. Congo red agar (CRA) and icaA and icaD genes by PCR were used for biofilm characterization. S. aureus was identified in 45.0% of samples. Resistance of S. aureus sample to antimicrobial was seen for penicillin (72.59%), erythromycin (51.85%), cefoxitin (20.74%), oxacillin (17.04%), clindamycin (14.81%) and levofloxacin (5.18%). MRSA was detected in 53.3% of the samples with SCCmec I (52.8%), SCCmec III (25%) and SCCmec IV (11.1%). Biofilm production was observed in 94.0% S. aureus samples. These data show that biosafety measures need to be enhanced in order to prevent dissemination of multiresistant and highly adhesive bacteria across other university settings, relatives, and close persons.

中文翻译:

从健康大学生的白大褂中分离出的金黄色葡萄球菌中抗生素敏感性和生物膜生成的测定。

这项研究旨在检测大学生白大褂中的金黄色葡萄球菌,并表征抗菌药的敏感性和生物膜的产生。从2015年8月至2017年3月从100名大学生的白大褂中获取细菌样本(n = 300)。分离金黄色葡萄球菌,并通过抗菌圆盘扩散技术评估其耐药性,筛选耐甲氧西林的金黄色葡萄球菌( MRSA),通过PCR检测mecA基因以及通过多重PCR测定葡萄球菌盒式染色体mec(SCCmec)。刚果红琼脂(CRA)以及icaA和icaD基因通过PCR进行生物膜表征。在45.0%的样品中鉴定出金黄色葡萄球菌。金黄色葡萄球菌样品对青霉素(72.59%),红霉素(51.85%),头孢西丁(20.74%),奥沙西林(17.04%),克林霉素(14.81%)和左氧氟沙星(5.18%)。在53.3%的SCCmec I(52.8%),SCCmec III(25%)和SCCmec IV(11.1%)样品中检测到MRSA。在94.0%的金黄色葡萄球菌样品中观察到生物膜产生。这些数据表明,需要加强生物安全措施,以防止在其他大学环境,亲戚和近亲中传播多抗性和高粘附力细菌。
更新日期:2020-04-22
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