In recent years, the chemical fingerprinting of traditional Chinese medicines and the metabolites in these compounds has been a hot topic. In the present study, the chemical fingerprint of Tianshu tablets (TST) and the metabolic characteristics of compounds in rats after intragastric administration were studied by ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC/Q-TOF MS). In a preliminary study, 77 chemical components in TST were determined by comparison with retention times, accurate molecular mass, and characteristic fragment ions of the known compounds in the literature and some well-known compounds were analyzed in detail, and the fragmentation pathways for parishins B, gastrodin A, and cnidilide or neocnilide were specifically analyzed. After intragastric administration of TST (4 g/kg) to rats, a total of 61 compounds were detected in plasma samples, including 7 prototypes and 54 metabolites. After further analysis, it was found that these metabolites were subjected to glucuronidation, sulfation, methylation, hydroxylation, dehydrogenation, or mixed metabolic processes. Hydroxylation and glucuronidation were finally confirmed as the main metabolic pathways. This is the first research on the chemical fingerprint and metabolites of TST, which lays a foundation for further investigation of TST.

中文翻译：

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超高效液相色谱/四极杆飞行时间质谱仪对天枢片的化学指纹图谱和代谢谱分析

近年来，中药的化学指纹图谱以及这些化合物中的代谢物已成为热门话题。本研究通过超高效液相色谱-四极杆飞行时间质谱（UPLC / Q-TOF MS）研究了天舒片（TST）的化学指纹图谱以及大鼠胃内给药后化合物的代谢特征。 ）。在初步研究中，通过与文献中已知化合物的保留时间，准确的分子质量和特征性碎片离子进行比较，确定了TST中的77种化学成分，并详细分析了一些知名化合物，并分析了帕里申斯的裂解途径B，天麻素A和cnidilide或neocnilide被专门分析。将TST（4 g / kg）灌胃给予大鼠后，血浆样品中总共检测到61种化合物，包括7种原型和54种代谢物。在进一步分析之后，发现这些代谢物经历了葡糖醛酸化，硫酸化，甲基化，羟基化，脱氢或混合代谢过程。最终确认了羟基化和葡萄糖醛酸化是主要的代谢途径。这是TST化学指纹图谱和代谢产物的首次研究，为进一步研究TST奠定了基础。最终确认了羟基化和葡萄糖醛酸化是主要的代谢途径。这是TST化学指纹图谱和代谢产物的首次研究，为进一步研究TST奠定了基础。最终确认了羟基化和葡萄糖醛酸化是主要的代谢途径。这是TST化学指纹图谱和代谢产物的首次研究，为进一步研究TST奠定了基础。