Objective. To explore the effects of sevoflurane on the latency and error times of the passive avoidance and levels of PSD-95 and AMPA receptors in the hippocampus. We evaluated the effects of sevoflurane on short-term memory in adult mice and explored the possible mechanism. Methods. 144 Kunming mice (2-3 months, 30-35 g) were randomly divided into two groups A () and B () and received the dark-avoidance (DA) and step-down avoidance (SA) tests, respectively. The groups DA and SA were further divided into control (inhaled 40% O₂ 2 h) and sevoflurane (3.3% sevoflurane and 40% O₂ 2 h) subgroups. Before inhalation intervention, all mice were trained to be familiar with the Morris water maze (MWM). According to the test points of behavioral indicators, 8 mice were randomly selected from each subgroup at point 12 h (T1), 24 h (T2), 48 h (T3), and 72 h (T4) after inhalation intervention. The step-through latency and error times were measured in 5 min. After the behavioral test, the mice were killed and the tissues of the hippocampus were taken for hematoxylin and eosin (H&E) staining. The expression level of PSD-95 and AMPA receptors in the hippocampus was detected by immunohistochemistry and Western Blot. The changes of synaptic transmission were measured via electrophysiology analysis of hippocampal slices. Results. The mice in the control subgroups found the platform in a shorter pathway than those in the sevoflurane subgroups during an MWM test. The step-through latency of T1 and T2 in the sevoflurane subgroup was shorter than baseline time, and the error times were increased in 5 min and higher than baseline time when compared with the control subgroup () in the A and B groups. Compared with the control subgroup, the expression level of PSD-95 and AMPA receptors in the hippocampus was decreased at T1 and T2 in the sevoflurane subgroup (). The nerve cells were partially swelling. Electrophysiology analysis showed that the levels of PSD-95 and AMPA receptor expression were associated with synaptic transmission. Conclusion. Sevoflurane impaired short-term memory in adult mice by inhibiting the expression of PSD-95 and AMPA receptors in the hippocampus, which led to the decrease in synaptic transmission.

中文翻译：

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七氟醚通过影响小鼠模型海马中的 PSD-95 和 AMPA 受体来损害短期记忆。

客观。探讨七氟醚对被动回避潜伏期和错误时间以及海马PSD-95和AMPA受体水平的影响。我们评估了七氟醚对成年小鼠短期记忆的影响，并探讨了可能的机制。方法。昆明小鼠144只（2-3个月，30-35 g）随机分为两组A（)和 B ()并分别接受避暗 (DA) 和降压避免 (SA) 测试。DA组和SA组进一步分为对照组（吸入40% O ₂ 2 h）和七氟醚组（3.3%七氟醚和40% O ₂2 h) 亚组。在吸入干预之前，所有小鼠都经过训练以熟悉莫里斯水迷宫 (MWM)。根据行为指标的测试点，在吸入干预后12 h（T1）、24 h（T2）、48 h（T3）和72 h（T4）各亚组随机抽取8只小鼠。步进延迟和错误时间在 5 分钟内测量。行为测试后，处死小鼠，取海马组织进行苏木精和伊红（H&E）染色。免疫组化和Western Blot检测海马PSD-95和AMPA受体的表达水平。通过海马切片的电生理分析测量突触传递的变化。结果. 在 MWM 测试期间，对照组中的小鼠发现该平台的通路比七氟醚亚组中的小鼠短。与对照组相比，七氟醚亚组T1和T2的逐步通过潜伏期短于基线时间，5 min内错误次数增加且高于基线时间（)在 A 组和 B 组中。与对照组相比，七氟醚组T1、T2海马PSD-95和AMPA受体表达水平降低。）。神经细胞部分肿胀。电生理分析显示PSD-95和AMPA受体表达水平与突触传递相关。结论。七氟醚通过抑制海马中 PSD-95 和 AMPA 受体的表达而损害成年小鼠的短期记忆，从而导致突触传递减少。