Abstract

Partial sequences of P-type ATPases were cloned from the marine microalgae Dunaliella maritima, two putative H⁺-ATPases (DmHA1 and DmHA2) and two putative Ca²⁺-ATPases (DmCA1 and DmCA2). The probable functions of the cloned proteins were suggested on the basis of their primary structure similarity with the proteins whose functions have been already characterized. The transcriptional response of the cloned D. maritima ATPase genes to a sharp increase in the NaCl concentration in the culture medium (from 100 to 500 mM) was investigated by quantitative RT-PCR. Hyperosmotic salt shock led to a significant increase in the DmHA2 expression and to a slight increase in the DmCA2 expression, whereas the expression of the two other ATPases, DmHA1 and DmCA1, was decreased. These data indicate that the DmHA2 ATPase is involved in maintenance of ion homeostasis in D. maritima cells under hyperosmotic salt shock.

中文翻译：

---

高渗盐冲击下海洋绿色微藻海藻P-型ATP酶的表达。

摘要

从海洋微藻Dunaliella maritima，两个推定的H ⁺ -ATPases（Dm HA1和Dm HA2）和两个推定的Ca ²⁺ -ATPases（Dm CA1和Dm CA2）克隆了P型ATPase的部分序列。根据克隆蛋白质与功能已被表征的蛋白质的一级结构相似性，提出了可能的功能。通过定量RT-PCR研究了克隆的海堤D. ATPase基因对培养基中NaCl浓度急剧增加（从100到500 mM）的转录反应。高渗盐溶液休克导致盐分显着增加Dm HA2表达和Dm CA2表达略有增加，而其他两个ATP酶Dm HA1和Dm CA1的表达则降低。这些数据表明，Dm HA2 ATPase在高渗盐冲击下参与了维氏梭菌细胞中离子稳态的维持。