Abstract

A new algorithm for computation of the initial velocity of enzyme reaction at the time zero is proposed. This algorithm makes it possible to reduce the systematic error of measurements to the minimum, to estimate the reaction velocity in test samples regardless of the enzyme activity levels, and to minimize the assay time. The study is illustrated by an example of salivary alpha-amylase and standard reagent kit. The algorithm should not be applied if conjugated enzyme systems are used because there is a long initial lag phase in the kinetic curve.

中文翻译：

---

一种更好地估计酶反应初始速度的方法。

摘要

提出了一种计算零时酶反应初始速度的新算法。该算法可以将测量的系统误差降低到最小，可以估计测试样品中的反应速度，而无需考虑酶的活性水平，并可以最大程度地缩短测定时间。唾液α-淀粉酶和标准试剂盒的例子说明了这项研究。如果使用共轭酶系统，则不应应用该算法，因为动力学曲线中存在较长的初始滞后阶段。