Abstract

The search for potential partners of human SELM in lysates of two cancer cell lines, HT-1080 (fibrosarcoma) and MCF-7 (breast adenocarcinoma), was carried out. Two cytoplasmic actin isoforms—cytoplasmic actin 1 (cytoskeleton β-actin) and cytoplasmic actin 2 (cytoskeletal γ-actin)—were identified as partners. In addition, the influence of two widely used antitumor selenium compounds (sodium selenite and methylseleninic acid) on the expression of SELM in cancer cells was studied. On the basis of the results obtained by real-time PCR and Western blotting, we concluded that 1 μM and 10 μM sodium selenite did not affect the expression of SELM in fibrosarcoma cells, whereas in breast adenocarcinoma cells 1 μM sodium selenite slightly increased expression and 10 μM sodium selenite significantly (approximately 2 times) decreased it. Methylseleninic acid in both cancer cell lines increased the SELM gene expression; the most pronounced effect was observed when fibrosarcoma cells were treated with 10 µM MSA (the expression of the hSelm gene increased almost 4 times).

中文翻译：

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硒蛋白SELM的蛋白质伴侣以及硒化合物在人类癌细胞中对其表达的调节中的作用。

摘要

在两种癌细胞系HT-1080（纤维肉瘤）和MCF-7（乳腺癌）的裂解物中寻找人SELM的潜在伴侣。鉴定了两个胞质肌动蛋白同工型-胞质肌动蛋白1（细胞骨架β-肌动蛋白）和胞质肌动蛋白2（细胞骨架γ-肌动蛋白）。此外，研究了两种广泛使用的抗肿瘤硒化合物（亚硒酸钠和甲基硒酸）对癌细胞中SELM表达的影响。根据实时PCR和Western印迹获得的结果，我们得出结论：1μM和10μM亚硒酸钠不会影响纤维肉瘤细胞中SELM的表达，而在乳腺腺癌细胞中1μM亚硒酸钠会略微增加表达和10μM亚硒酸钠显着降低（约2倍）。两种癌细胞系中的甲基硒酸均增加了SELM基因的表达。当用10 µM MSA处理纤维肉瘤细胞时，观察到最明显的效果（hSelm基因几乎增加了4倍。