Control of the spatial distribution of various cell types is required to construct functional tissues. Here, we report a simple topographical structure changed the spatial cell density. A concave curved boundary was designed, which allowed the spatial descent moving of cells and the change in spatial distributions of co-cultured cells. We utilized the difference in cell motility between myoblast cells (C2C12) and neuronal cells (PC12) to demonstrate the feasibility of spontaneous change in spatial cell density. Without the curved boundaries, high motility cells (C2C12) did not migrate to the adjacent area, which resulted in a slight temporal change (< 15%) in the spatial cell distribution. In contrast, with the curved boundaries, the cell density of the high motility cells in the groove to those cells on the ridge showed an increase exceeding 45%. On the other hand, the temporal change in the spatial cell distribution of low motility cells (PC12) was below 15% with or without the curved boundaries. In addition, as groove width increased, both cells displayed more initially gathering in groove. Importantly, these cell-type dependent results were also maintained under co-culture conditions. Our results suggest that designing topographical interfaces changes spatial cell density without any manipulation and is useful for multi-cellular constructs.

中文翻译：

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设计的地形结构，可使用细胞迁移来控制空间细胞密度。

需要控制各种细胞类型的空间分布以构建功能组织。在这里，我们报告一个简单的地形结构改变了空间像元密度。设计了凹形弯曲边界，其允许细胞的空间下降运动和共培养细胞的空间分布变化。我们利用成肌细胞（C2C12）和神经元细胞（PC12）之间的细胞运动差异来证明空间细胞密度自发改变的可行性。没有弯曲的边界，高运动力细胞（C2C12）不会迁移到相邻区域，这导致空间细胞分布发生轻微的时间变化（<15％）。相反，在弯曲的边界处，沟中高运动细胞的细胞密度相对于上那些细胞的细胞密度增加了超过45％。另一方面，在有或没有弯曲边界的情况下，低运动性细胞（PC12）的空间细胞分布的时间变化低于15％。另外，随着凹槽宽度的增加，两个单元最初显示的更多地聚集在凹槽中。重要的是，这些细胞类型依赖性结果也可以在共培养条件下保持。我们的结果表明，设计地形界面可以改变空间细胞密度，而无需任何操作，对于多细胞构造很有用。最初，两个单元都显示出更多地聚集在凹槽中。重要的是，这些细胞类型依赖性结果也可以在共培养条件下保持。我们的结果表明，设计地形界面可以改变空间细胞密度，而无需任何操作，对于多细胞结构很有用。最初，两个单元都显示更多地聚集在凹槽中。重要的是，这些细胞类型依赖性结果也可以在共培养条件下保持。我们的结果表明，设计地形界面可以改变空间细胞密度，而无需任何操作，对于多细胞结构很有用。