African swine fever (ASF) is a fatal disease caused by a virus in domestic pigs. In this study, a real-time loop-mediated isothermal amplification (LAMP) assay and visual LAMP assay were developed for the detection of African swine fever virus (ASFV). LAMP primers targeting the p10 gene of ASFV were designed, the LAMP reaction system was optimized with plasmid pUC57 containing the p10 gene sequence, and the specificities of the real-time LAMP and the visual assays were tested with the DNA or cDNA of pseudorabies virus (PRV), porcine circovirus type 2 (PCV2), classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine parvovirus (PPV) and ASFV, as well as the plasmid pUC57 containing the p10 gene sequence. The detection limits were determined using a serial dilution of plasmid pUC57 containing the p10 gene sequence. Our results showed that the LAMP assays could accurately and specifically detect ASFV with a detection limit of 30 copies per μl-1 of pUC57 containing p10 gene sequence. In addition, the LAMP assays were further evaluated using various genotypes of ASFV strains. Furthermore, the LAMP assays are comparable with the well-established real-time PCR assay. This study provides promising solutions for facilitating preliminary and cost-effective surveillance for prevention and control of ASFV.

中文翻译：

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实时环介导的等温扩增（LAMP）分析和视觉LAMP分析的开发，用于检测非洲猪瘟病毒（ASFV）。

非洲猪瘟（ASF）是由家猪中的病毒引起的致命疾病。在这项研究中，实时环介导的等温扩增（LAMP）测定法和视觉LAMP测定法被开发用于检测非洲猪瘟病毒（ASFV）。设计了针对ASFV p10基因的LAMP引物，用包含p10基因序列的质粒pUC57优化了LAMP反应系统，并用伪狂犬病病毒的DNA或cDNA检测了实时LAMP的特异性和目测法（ PRV），2型猪圆环病毒（PCV2），经典猪瘟病毒（CSFV），猪繁殖与呼吸综合征病毒（PRRSV），猪细小病毒（PPV）和ASFV以及含有p10基因序列的质粒pUC57。使用含有p10基因序列的质粒pUC57的系列稀释液确定检测限。我们的结果表明，LAMP分析可以准确，特异性地检测ASFV，检测极限为每μl-1含p10基因序列的pUC57 30拷贝。此外，使用各种基因型的ASFV菌株进一步评估了LAMP分析。此外，LAMP分析可与公认的实时PCR分析相媲美。这项研究为促进对ASFV的预防和控制进行初步且具有成本效益的监视提供了有希望的解决方案。使用各种基因型的ASFV菌株进一步评估LAMP分析。此外，LAMP分析可与公认的实时PCR分析相媲美。这项研究为促进对ASFV的预防和控制进行初步且具有成本效益的监视提供了有希望的解决方案。使用各种基因型的ASFV菌株进一步评估LAMP分析。此外，LAMP分析可与公认的实时PCR分析相媲美。这项研究为促进对ASFV的预防和控制进行初步且具有成本效益的监视提供了有希望的解决方案。