Catalytic transformation of biomass-derived furans into value-added chemicals and biofuels has received considerable interest recently. In this work, aldehyde dehydrogenases (ALDHs) were identified from Comamonas testosteroni SC1588 for the oxidation of bio-based furans into furan carboxylic acids. Of the whole-cell biocatalysts constructed, Escherichia coli expressing a vanillin dehydrogenase (E. coli_CtVDH1) proved to be the best for the oxidation of 5-hydroxymethylfurfural (HMF). 5-Hydroxymethyl-2-furancarboxylic acid (HMFCA) was obtained in the yield of approximately 92 % within 12 h using this recombinant strain when the HMF concentration was up to 200 mM. In a fed-batch process, 292 mM of HMFCA was produced within 20.5 h, thereby providing a productivity as high as 2.0 g/L h. Other furan carboxylic acids were synthesized in the yields of 83-95%. Besides, the partially purified HMF was smoothly converted into HMFCA by this recombinant strain, with a 90% yield.

中文翻译：

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过表达醛脱氢酶的大肠杆菌有效合成5-羟甲基-2-呋喃甲酸。

最近，将生物质衍生的呋喃催化转化为增值化学品和生物燃料的兴趣很大。在这项工作中，从Comamonas testosteroni SC1588中鉴定出醛脱氢酶（ALDHs），用于将生物基呋喃氧化为呋喃羧酸。在构建的全细胞生物催化剂中，表达香草醛脱氢酶的大肠杆菌（大肠杆菌_CtVDH1）被证明是氧化5-羟甲基糠醛（HMF）的最佳方法。当HMF浓度高达200 mM时，使用该重组菌株，在12 h内以约92％的产率获得了5-羟甲基-2-呋喃甲酸（HMFCA）。在分批补料工艺中，在20.5小时内产生了292 mM的HMFCA，从而提供了高达2.0 g / L h的生产率。合成其他呋喃羧酸，收率为83-95％。此外，通过该重组菌株将部分纯化的HMF平稳地转化为HMFCA，产率为90％。