The Centers for Disease Control and Prevention, Arbovirus Reference Collection (ARC) contains viral isolates from both environmental and human sources that are maintained in the laboratory through passage in suckling mouse brain and/or vertebrate and invertebrate cell culture. There has been increased concern regarding the effect of mycoplasma contamination on virus growth and its impact on research and phenotypic analysis. Therefore, quality control testing of virus preparations has become a routine part of the ARC quality assurance program. We compared the performance of three kits - the PCR Mycoplasma Detection Kit (ABM), the VenorGem Mycoplasma Detection Kit (Sigma), and the MycoAlert Mycoplasma Detection Kit (Lonza) - against a reference mycoplasma detection assay from the American Tissue Culture Collection (ATCC) using 744 virus preparations in the ARC, representing 721 unique viruses comprising twelve families and unclassified viruses. We found the ABM kit had the highest sensitivity and specificity, followed by the Sigma kit and Lonza kit, when compared to the ATCC kit. An increase in false positives was observed for the Lonza kit for preparations recently passaged in suckling mouse. Our data supports previously reported observations; that once introduced a specific species of mycoplasma is maintained within a lab.

中文翻译：

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历史虫媒病毒存储库中的支原体检测：商业试剂盒比较及其对改进存储库管理的意义。

疾病控制和预防中心，虫媒病毒参考资料库（ARC）包含来自环境和人类来源的病毒分离物，这些分离物通过在乳鼠大脑和/或脊椎动物和无脊椎动物细胞培养物中的传代而保存在实验室中。人们越来越关注支原体污染对病毒生长的影响及其对研究和表型分析的影响。因此，病毒制剂的质量控制测试已成为ARC质量保证计划的常规组成部分。我们比较了三种试剂盒的性能-PCR支原体检测试剂盒（ABM），VenorGem支原体检测试剂盒（Sigma），和MycoAlert支原体检测试剂盒（Lonza）-针对美国组织培养物保藏中心（ATCC）的参考支原体检测测定法，使用ARC中的744种病毒制剂，代表721种独特病毒，包括十二个家族和未分类病毒。我们发现，与ATCC试剂盒相比，ABM试剂盒具有最高的灵敏度和特异性，其次是Sigma试剂盒和Lonza试剂盒。对于最近在乳鼠中传代的制剂，Lonza试剂盒观察到假阳性的增加。我们的数据支持先前报告的观察结果；一旦引入了特定种类的支原体，就可以在实验室内进行维护。与ATCC套件相比，其次是Sigma套件和Lonza套件。对于最近在乳鼠中传代的制剂，Lonza试剂盒观察到假阳性的增加。我们的数据支持先前报告的观察结果；一旦引入了特定种类的支原体，便会在实验室内进行维护。与ATCC套件相比，其次是Sigma套件和Lonza套件。对于最近在乳鼠中传代的制剂，Lonza试剂盒观察到假阳性的增加。我们的数据支持先前报告的观察结果；一旦引入了特定种类的支原体，便会在实验室内进行维护。