Carboxylic acid reductase (CAR) enzymes are large multi-domain proteins that catalyse the ATP- and NADPH-dependent reduction of wide range of acids to the corresponding aldehydes. This particular reaction is of considerable biotechnological interest. Recent advances in the structural and solution studies of isolated domain, di-domain and full-length CAR enzymes revealed valuable insights into the mechanism of carboxylic acid reduction activity. This review features the phylogenetic, sequence and structural insight into the CAR and implications of these observations in order to improve carboxylic acid reduction activity to develop CAR as robust biocatalyst.

中文翻译：

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羧酸还原酶：结构和机理。

羧酸还原酶（CAR）酶是大型的多域蛋白，可催化ATP和NADPH依赖性的多种酸还原为相应的醛。该特定反应具有相当大的生物技术意义。分离域，双域和全长CAR酶的结构和溶液研究的最新进展揭示了对羧酸还原活性机理的宝贵见解。这篇综述介绍了CAR的系统发育，序列和结构洞察力，以及这些观察结果的意义，目的是提高羧酸还原活性，从而将CAR发展成为强大的生物催化剂。