Exosomes, secreted by various cells, are nanometer-scale vesicles with the functions in intercellular communication. To understand a role of exosomal miRNAs in the sheeppox virus infection, exosomes were isolated from sheeppox virus-infected sheep testicular cells 0 h, 24 h and 72 h post infection. The results of transmission electron microscopy and size distribution showed that all three exosome samples were spherical particles with negatively-stained membrane, ranging from 39 nm to 127 nm in diameter. A total of 106 known and 279 novel miRNAs were identified, and 78 known and 54 novel miRNAs were commonly detected in three exosome samples. Compared with the exosomes by the uninfected controls, a total of 34 known miRNAs were aberrantly expressed in the exosomes from infected cells. In agreement with the sequencing data, the expression of oar-miR-21 and oar-miR-10b was shown to be the highest in exosomes at 24 h after SPPV-infected, and the expression of oar-let-7f was the highest in exosomes at 72 h. Conversely, the expression of oar-let-7b and oar-miR-221 was significantly decreased 24 h and 72 h post infection compared with 0 h. The analysis results also revealed that differentially expressed miRNAs were mostly involved in an immune system process and stimulus response. These results provide rich data to further investigate a role of exosomal miRNAs in SPPV-host interactions.

中文翻译：

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羊痘病毒感染的绵羊睾丸细胞释放的外泌体中miRNA的比较分析。

由各种细胞分泌的外来体是具有细胞间通讯功能的纳米级囊泡。为了了解外泌体miRNA在绵羊痘病毒感染中的作用，在感染后0 h，24 h和72 h从绵羊痘病毒感染的绵羊睾丸细胞中分离了外泌体。透射电子显微镜和尺寸分布的结果表明，所有三个外泌体样品均为球形颗粒，带有负染色膜，直径范围为39 nm至127 nm。总共鉴定出106种已知的miRNA和279种新颖的miRNA，并且在三个外泌体样品中通常检测到78种已知的miRNA和54种新颖的miRNA。与未感染对照的外泌体相比，来自感染细胞的外泌体中共表达了34种已知的miRNA。与测序数据一致，SPPV感染后24 h，oar-miR-21和oar-miR-10b的表达最高，而72 h时oar-let-7f的表达最高。相反，感染后24 h和72 h，oar-let-7b和oar-miR-221的表达与0 h相比明显降低。分析结果还表明，差异表达的miRNA主要参与免疫系统过程和刺激反应。这些结果提供了丰富的数据，可进一步研究外泌体miRNA在SPPV-宿主相互作用中的作用。分析结果还表明，差异表达的miRNA主要参与免疫系统过程和刺激反应。这些结果提供了丰富的数据，可进一步研究外泌体miRNA在SPPV-宿主相互作用中的作用。分析结果还表明，差异表达的miRNA主要参与免疫系统过程和刺激反应。这些结果提供了丰富的数据，可进一步研究外泌体miRNA在SPPV-宿主相互作用中的作用。