Malassezia spp. are commensal yeasts that can cause cutaneous ailments such as dandruff and seborrheic dermatitis. We sought to develop a cost-effective, herbal formulation for the treatment of cutaneous ailments related to Malassezia spp. Aqueous and ethanolic extracts of fenugreek (Trigonellafoenum-graecum L.) leaves exhibited activity against a clinical isolate and commercial strain of Malassezia furfur. The extracts were also found to be active against other pathogenic fungi such as Aspergillus niger and Candida albicans. Qualitative and quantitative phytochemical evaluation of aqueous extract showed a predominant presence of flavonoids apart from alkaloids, saponins, carbohydrates, phenols, and proteins. Gel formulation of 30% aqueous fenugreek leaf extract was developed and optimized using sodium alginate as a gelling agent. The formulation showed good physicochemical characteristics and retained activity against M. furfur during 3-month accelerated stability studies. Furthermore, the developed herbal gel formulation did not show any irritation or sensitization in New Zealand rabbits after topical application, proving its cutaneous safety. Thus, topical gel formulation containing fenugreek leaf aqueous extract could be a safe and effective herbal treatment for various cutaneous fungal infections, including dandruff.

中文翻译：

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胡芦巴叶提取物及其凝胶制剂对糠Mal马拉色菌具有活性。

马拉色菌属。是常见的酵母菌，可引起皮肤病，例如头皮屑和脂溢性皮炎。我们寻求开发一种经济有效的草药配方，以治疗与马拉色菌属有关的皮肤病。胡芦巴（Trigonellafoenum-graecum L.）叶片的水和乙醇提取物表现出对糠isolate马拉色氏菌的临床分离株和商业菌株的活性。还发现提取物对其他病原真菌如黑曲霉和白色念珠菌具有活性。水提取物的定性和定量植物化学评估显示，除生物碱，皂角苷，碳水化合物，酚和蛋白质外，类黄酮的主要存在。使用藻酸钠作为胶凝剂，开发并优化了30％胡芦巴叶提取物的凝胶配方。在3个月的加速稳定性研究中，该制剂显示出良好的理化特性并保留了对抗糠M分支杆菌的活性。此外，在局部应用后，所开发的草药凝胶制剂在新西兰兔子中未显示任何刺激或致敏性，证明了其皮肤安全性。因此，含有胡芦巴叶水提取物的局部凝胶制剂对于包括头皮屑在内的各种皮肤真菌感染可能是一种安全有效的草药疗法。