BACKGROUND Plumbagin is one of the pharmaceutically important biomolecule with anticancer potential. Among the plants reported to produce plumbagin, P. zeylanica topped the list. The plumbagin production is very slow with low yield and maximum 0.5% (of dry weight) was reported in P. zeylanica. To meet the increasing demand of the plumbagin at global level, the P. zeylanica are exploited at commercial level, which may pose serious threat on the germplasm of the plant populations. So, it is needed to enhance the contents of plumbagin in P. zeylanica using biotechnological approaches. Among the various methods used to enhance the contents of plumbagin in P. zeylanica, utilization of fungal endophytes to enhance the plumbagin contents is a widely accepted approach. As fungal endophytes have the potential to synthesize various secondary metabolites and also reported to influence the synthesis of the secondary metabolites in plants. In the present study, an attempt was made to assess the effect of fungal endophytes of the Plumbago zeylanica L. on enhancement of plumbagin contents at in vivo level. RESULTS Total 3 fungal endophytes were recorded from the roots of P. zeylanica collected from Khadki, Pune. The fungal endophytes were identified at morphological and molecular level. After 1 year of the treatment with fungal endophytes, significant enhancement of plumbagin was recorded in the roots of the P. zeylanica. Plumbagin contents in each were quantified against the standard plumbagin by employing LCMS-MS technique. Among the three fungal endophytes, the maximum enhancement of plumbagin content (122.67%) was reported with the treatment of Alternaria sp. (Isolate-3) in the roots of the P. zeylanica compared to control. CONCLUSION Among the three fungal endophytes, the maximum enhancement of plumbagin content (122.67%) was reported with Alternaria sp. (Isolate 3) in the roots of the pot-grown plants of P. zeylanica at in vivo level.

中文翻译：

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西洋李的真菌内生菌增加了西洋参的含量。

背景技术铅白蛋白是具有抗癌潜力的药学上重要的生物分子之一。在据报道可生产plumbagin的植物中，P。zeylanica居首位。羽扇豆的生产非常缓慢，产量很低，据报道，玉米中最大含量为干重的0.5％。为了满足全球范围内对白羽烟袋日益增长的需求，在商业层面开发了玉米lan，这可能对植物种群的种质构成严重威胁。因此，有必要利用生物技术方法提高玉米ze中铅皮素的含量。在用于增加zeylanica的plumbagin含量的各种方法中，利用真菌内生菌提高plumbagin含量是一种广泛接受的方法。由于真菌内生菌具有合成各种次生代谢产物的潜力，并且据报道也影响植物中次生代谢产物的合成。在本研究中，人们试图在体内水平上评估西洋李的真菌内生菌对增加铅袋素含量的影响。结果记录了从浦那Khadki收集的zeylanica根的共3种真菌内生菌。在形态和分子水平上鉴定了真菌内生菌。用真菌内生菌处理1年后，在zeylanica的根中记录了plumbagin的显着增强。通过使用LCMS-MS技术，相对于标准李子白蛋白定量每种中李子白蛋白的含量。在三种真菌内生菌中，李子苷的含量最大程度提高（122。67％）被报道了链格孢菌的治疗。与对照相比，玉米。根中的（分离物3）。结论在3种真菌内生菌中，报道了链格孢菌（Alternaria sp。）铅蛋白的最大增加（122.67％）。（分离物3）在体内水平的P. zeylanica盆栽植物的根中。