Abstract The emergence and spread of multidrug-resistant (MDR) pathogenic bacteria is a clinical problem that requires novel anti-infective agents. Targeting pathogenic biofilms is considered a promising strategy to control bacterial infections. In this study, bioactive extracts of Carum copticum were investigated for their anti-biofilm efficacy against extended spectrum β-lactamase (ESβL) producing MDR enteric bacteria. Thymol was also tested for its anti-biofilm properties, as gas chromatography-mass spectrometry revealed a high content (65.8%) of this phytochemical in the C. copticum methanolic extract. Biofilm inhibition was assessed in microtitre plates and further validated by light, electron and confocal laser microscopy. Sub-inhibitory concentrations of bioactive extracts of C. copticum and thymol significantly prevented biofilm development, ranging from 78.6 to 83.9% reductions. Microscopic analysis revealed that biofilms made by ESβL producing MDR enteric bacteria had a weakened structure, scattered microcolonies, and reduced cell density and thickness after exposure to the bioactive extracts and thymol.

中文翻译：

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Carum copticum 和百里酚的生物活性提取物通过产生多重耐药的超广谱 β-内酰胺酶肠道细菌抑制生物膜的发展

摘要 多重耐药（MDR）病原菌的出现和传播是一个需要新型抗感染药物的临床问题。靶向病原生物膜被认为是控制细菌感染的有前途的策略。在这项研究中，研究了香芹的生物活性提取物对产生超广谱 β-内酰胺酶 (ESβL) 的 MDR 肠道细菌的抗生物膜功效。还测试了百里酚的抗生物膜特性，因为气相色谱-质谱分析显示，在 C.copticum 甲醇提取物中，这种植物化学物质的含量很高 (65.8%)。在微量滴定板上评估生物膜抑制，并通过光、电子和共聚焦激光显微镜进一步验证。C. 生物活性提取物的亚抑制浓度。科普特和百里酚显着阻止了生物膜的发展，减少了 78.6% 到 83.9%。显微分析表明，在暴露于生物活性提取物和百里酚后，产生 ESβL 的 MDR 肠道细菌形成的生物膜结构减弱，微菌落分散，细胞密度和厚度降低。