Decellularized adipose tissue (DAT) is a promising biomaterial for adipose tissue engineering. However, there is a lack of research of DAT prepared from xenogeneic porcine adipose tissue. This study aimed to compare the adipogenic ability of DAT derived from porcine subcutaneous (SDAT) and visceral adipose tissue (VDAT). The retention of key collagen in decellularized matrix was analysed to study the biochemical properties of SDAT and VDAT. For the biomechanical study, both DAT materials were fabricated into three-dimensional (3D) porous scaffolds for rheology and compressive tests. Human adipose-derived stem cells (ADSCs) were cultured on both scaffolds to further investigate the effect of matrix stiffness on cellular morphology and on adipogenic differentiation. ADSCs cultured on soft VDAT exhibited significantly reduced cellular area and upregulated adipogenic markers compared to those cultured on SDAT. In vivo results revealed higher adipose regeneration in the VDAT compared to the SDAT. This study further demonstrated that the relative expression of collagen IV and laminin was significantly higher in VDAT than in SDAT, while the collagen I expression and matrix stiffness of SDAT was significantly higher in comparison to VDAT. This result suggested that porcine adipose tissue could serve as a promising candidate for preparing DAT.

中文翻译：

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猪皮下和内脏脂肪组织脱细胞支架的生化和生物力学比较。

去细胞脂肪组织（DAT）是用于脂肪组织工程的有前途的生物材料。然而，缺乏从异种猪脂肪组织制备的DAT的研究。这项研究旨在比较源自猪皮下（SDAT）和内脏脂肪组织（VDAT）的DAT的成脂能力。分析了脱细胞基质中关键胶原的保留，以研究SDAT和VDAT的生化特性。为了进行生物力学研究，将两种DAT材料都制成了三维（3D）多孔支架，用于流变学和抗压测试。在两个支架上培养人脂肪干细胞（ADSC），以进一步研究基质刚度对细胞形态和成脂分化的影响。与在SDAT上培养的ADSC相比，在软VDAT上培养的ADSC的细胞面积显着减少，并且成脂标记物上调。体内结果显示，与SDAT相比，VDAT中的脂肪再生更高。这项研究进一步证明，VDAT中胶原蛋白IV和层粘连蛋白的相对表达显着高于SDAT，而SDAT中胶原蛋白I的表达和基质硬度比VDAT显着更高。该结果表明，猪脂肪组织可以作为制备DAT的有希望的候选者。这项研究进一步证明，VDAT中胶原蛋白IV和层粘连蛋白的相对表达显着高于SDAT，而SDAT中胶原蛋白I的表达和基质硬度比VDAT显着更高。该结果表明，猪脂肪组织可以作为制备DAT的有希望的候选者。这项研究进一步证明，VDAT中胶原蛋白IV和层粘连蛋白的相对表达显着高于SDAT，而SDAT中胶原蛋白I的表达和基质硬度比VDAT显着更高。该结果表明，猪脂肪组织可以作为制备DAT的有希望的候选者。