Microsporidia are intracellular fungal parasites and they are the most common pathogens for sericulture. Microsporidian sp. can cause pebrine, a dreadful disease and lead to destructive disorder in Muga silkworm, Antheraea assamensis Helfer by vertical and horizontal transmission. This disease is the key factor obstructing the developmental progress of Muga culture in India. Nevertheless, molecular identification and characterization of pathogen associated with pebrine disease in A. assamensis is not yet established. Insect bioassay studies revealed that microsporidian infection in Muga silkworm, A. assamensis Helfer significantly reduced (P < 0.005) cocoon weight, pupal weight, shell weight and silk ratios. A new set of PCR primers suitable for amplification of small subunit ribosomal RNA (SSU-rRNA) of microsporidia infecting A. assamensis have been designed. The amplicon was cloned, sequenced and analysed. Microsporidia pathogen of wild silk moth A. assamensis has been identified at genus level as Nosema sp. AA1. Phylogeny of Nosema sp. AA1 was constructed on the basis of SSU-rRNA sequence and it has a close evolutionary relationship with microsporidian pathogens of other wild silkmoths. The arrangement and organization of the rRNA genes inferred that Nosema sp. AA1 belongs to true Nosema group and not to members of the Nosema/Vairimorpha group.

中文翻译：

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导致No虫蚕No蛾致病的微孢子虫病原菌的分离和分子鉴定。

微孢子虫是细胞内的真菌寄生虫，它们是蚕桑最常见的病原体。微孢子虫 可引起微粒子，一种可怕的疾病，并导致在木嘎蚕，破坏性病症天蚕assamensis Helfer由垂直和水平传输。该病是阻碍印度穆加文化发展的关键因素。然而，分子鉴定，并与微粒子病病原相关表征A. assamensis尚未建立。昆虫生物测定研究表明，穆加蚕（A. assamensis Helfer）中的微孢子虫感染显着减少（P <0.005）茧重，p重，壳重和蚕丝比。一组新的PCR引物适合的微孢子感染小亚基核糖体RNA（SSU-rRNA基因）的扩增A. assamensis已经设计。扩增子被克隆，测序和分析。野生蚕蛾A.amsamensis的小孢子虫病原体已被鉴定为属Nosema sp.。AA1。Nosema sp。的系统发育。AA1是在SSU-rRNA序列的基础上构建的，与其他野生蛾类的微孢子虫病原体有着密切的进化关系。rRNA基因的排列和组织推断Nosem a sp。AA1属于真正的Nosema组，而不是Nosema / Vairimorpha组的成员。